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Title: | Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity in Equisetum telmateia | Authors: | Correia, Helena González-Paramás, Ana Amaral, Maria Teresa Santos-Buelga, Celestino Batista, Maria Teresa |
Issue Date: | 2005 | Citation: | Phytochemical Analysis. 16:5 (2005) 380-387 | Abstract: | The antioxidant activity of an aqueous extract (infusion) and respective ethyl acetate fraction of Equisetum telmateia Ehrh. (Equisetaceae), a plant used in traditional medicine for its anti-inflammatory and diuretic properties, has been evaluated by DPPH, TEAC and TBARS assays. A high and significant antioxidant activity was detected in the ethyl acetate fraction. Analysis of the aqueous extract and the ethyl acetate fraction by HPLC-PAD-ESI/MS allowed the identification of the major phenolic compounds as flavan-3-ol, kaempferol and phenolic acid derivatives. Among the flavan-3-ols, A-type proanthocyanidins and afzelechin derivatives were detected as well as the more common B-type procyanidins, B2 and C1, whose identification was further confirmed by HPLC using detection involving chemical reaction with p-dimethylamino-cinnamaldehyde. The results suggest that the anti-inflammatory activity of E. telmateia could be due, at least in part, to the presence of compounds with antioxidant activity. Copyright © 2005 John Wiley & Sons, Ltd. | URI: | https://hdl.handle.net/10316/8378 | DOI: | 10.1002/pca.864 | Rights: | openAccess |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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