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Title: | Chlorotetracycline As An Indicator Of The Interaction Of Calcium With Brain Membrane Fractions | Authors: | Carvalho, C. A. M. | Issue Date: | 1978 | Citation: | Journal of Neurochemistry. 30:5 (1978) 1149-1155 | Abstract: | The fluorescence of chlorotetracycline (CTC) in the presence of synaptosomes isolated from sheep brain is selectively increased by Ca2+ under conditions in which Mg2+, Na+, K+, Li+ or choline have only a small effect. The monovalent cations release bound Ca2+ from synaptosomes, and this effect is reflected by a decrease in the CTC fluorescence. Under optimal conditions there is a near parallelism between Ca2+ and CTC binding to the synaptosomes membranes, and Li+ is the monovalent cation tested which interferes the most with the binding of both substances. These results obtained in a predominantly sucrose medium become less distinct when media simulating physiological composition are utilized, which limits the usefulness of the method. Brain mitochondria and myelin also bind Ca2+ and CTC. The ratio of the fluorescence signal (or CTC bound) to Ca2+ bound is highest of all for mitochondrial membranes, and the apparent fluorescence quantum yield of CTC is also the highest in these membranes, which suggests that the Ca2+ in these membranes is localized in a more apolar region than is the case for synaptosomes and myelin. | URI: | https://hdl.handle.net/10316/8346 | DOI: | 10.1111/j.1471-4159.1978.tb12410.x | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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