Please use this identifier to cite or link to this item:
|Title:||Effect of amyloid beta-peptide on permeability transition pore: A comparative study||Authors:||Moreira, Paula I.
Santos, Maria S.
Rego, A. Cristina
|Issue Date:||2002||Citation:||Journal of Neuroscience Research. 69:2 (2002) 257-267||Abstract:||A potentially central factor in neurodegeneration is the permeability transition pore (PTP). Because of the tissue-specific differences in pore properties, we directly compared isolated brain and liver mitochondria responses to the neurotoxic Abeta peptides. For this purpose, the following parameters were examined: mitochondrial membrane potential (DeltaPsim), respiration, swelling, ultrastructural morphology, and content of cytochrome c. Both peptides, Abeta25-35 (50 muM) and Abeta1-40 (2 muM), had a similar toxicity, exacerbating the effects of Ca2+, although, per se, they did not induce (PTP). In liver mitochondria, Abeta led to a drop in DeltaPsim and potentiated matrix swelling and disruption induced by Ca2+. In contrast, brain mitochondria, exposed to the same conditions, demonstrated a higher capacity to accumulate Ca2+ before the DeltaPsim drop and a slight increase of mitochondrial swelling compared with liver mitochondria. Furthermore, mitochondrial respiratory state 3 was depressed in the presence of Abeta, whereas state 4 was unaltered, resulting in an uncoupling of respiration. In both types of mitochondria, Abeta did not affect the content of cytochrome c. The DeltaPsim drop was reversed when Ca2+ was removed by EGTA or when ADP plus oligomycin was present. Pretreatment with cyclosporin A or ADP plus oligomycin prevented the deleterious effects promoted by Abeta and/or Ca2+. It can be concluded that brain and liver mitochondria show a different susceptibility to the deleterious effect of Abeta peptide, brain mitochondria being more resistant to the potentiation by Abeta of Ca2+-induced PTP. © 2002 Wiley-Liss, Inc.||URI:||http://hdl.handle.net/10316/8324||DOI:||10.1002/jnr.10282||Rights:||openAccess|
|Appears in Collections:||FCTUC Ciências da Vida - Artigos em Revistas Internacionais|
Show full item record
checked on Feb 18, 2020
WEB OF SCIENCETM
checked on Nov 7, 2019
Page view(s) 5871
checked on Apr 2, 2020
checked on Apr 2, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.