Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8147
Title: Comparative effects of herbicide dicamba and related compound on plant mitochondrial bioenergetics
Authors: Peixoto, Francisco 
Vicente, Joaquim A. F. 
Madeira, Vítor M. C. 
Issue Date: 2003
Citation: Journal of Biochemical and Molecular Toxicology. 17:3 (2003) 185-192
Abstract: The herbicide dicamba (3,6-dichloro-2-methoxybenzoic acid) was evaluated for its effects on bioenergetic activities of potato tuber mitochondria to elucidate putative mechanisms of action and to compare its toxicity with 2-chlorobenzoic acid. Dicamba (4 mumol/mg mitochondrial protein) induces a limited stimulation of state 4 respiration of ca. 10%, and the above concentrations significantly inhibit respiration, whereas 2-chlorobenzoic acid maximally stimulates state 4 respiration (ca. 50%) at about 25 mumol/mg mitochondrial protein. As opposed to these limited effects on state 4 respiration, transmembrane electrical potential is strongly decreased by dicamba and 2-chlorobenzoic acid. Dicamba (25 mumol/mg mitochondrial protein) collapses, almost completely, Deltapsi; similar concentrations of 2-chlorobenzoic acid promote Deltapsi drops of about 50%. Proton permeabilization partially contributes to Deltapsi collapse since swelling in K-acetate medium is stimulated, with dicamba promoting a stronger stimulation. The Deltapsi decrease induced by dicamba is not exclusively the result of a stimulation on the proton leak through the mitochondrial inner membrane, since there was no correspondence between the Deltapsi decrease and the change on the O2 consumption on state 4 respiration; on the contrary, for concentrations above 8 mumol/mg mitochondrial protein a strong inhibition was observed. Both compounds inhibit the activity of respiratory complexes II and III but complex IV is not significantly affected. Complex I seems to be sensitive to these xenobiotics. In conclusion, dicamba is a stronger mitochondrial respiratory chain inhibitor and uncoupler as compared to 2-chlorobenzoic acid. Apparently, the differences in the lipophilicity are related to the different activities on mitochondrial bioenergetics. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:185-192, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10077
URI: http://hdl.handle.net/10316/8147
DOI: 10.1002/jbt.10077
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
obra.pdf114.17 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

1
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations

2
checked on Feb 18, 2020

Page view(s)

138
checked on May 25, 2020

Download(s)

68
checked on May 25, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.