Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8135
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Francisco, R. | - |
dc.contributor.author | Alpoim, M. C. | - |
dc.contributor.author | Morais, P. V. | - |
dc.date.accessioned | 2009-02-09T11:10:33Z | - |
dc.date.available | 2009-02-09T11:10:33Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Journal of Applied Microbiology. 92:5 (2002) 837-843 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8135 | - |
dc.description.abstract | Aims: This study attempts to establish a relationship between the Cr(VI) resistance of the culturable microbial community and the Cr(VI) resistance and Cr(VI)-reducing ability of representative strains of each population, in order to assess whether these are exclusive characteristics of one microbial group or abilities shared among many groups. Methods and Results: A group of 48 Cr(VI)-resistant isolates, with different colony types, was isolated from chromium-contaminated activated sludge. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis protein patterns and fatty acid methyl ester analysis identified six populations, representing 54% of the isolated bacteria, as belonging to the genera Acinetobacter and Ochrobactrum. The remaining populations included strains identified as species of the 03B2-Proteobacteria and high G + C Gram-positive bacteria. The Cr(VI) resistance and reduction ability of the strains were tested. All but two isolates grew in the presence of 1 mmol l22121 Cr(VI). During enrichment, all isolates were able to survive to 2 mmol l22121 Cr(VI) and complete Cr(VI) reduction was achieved. Representative strains of each population were able to partially reduce (5·4201339·1%) the Cr(VI) present in the growth medium. Conclusions: Most of the identified isolates have never been reported to be Cr(VI)-resistant and/or Cr(VI)-reducing strains. The mechanisms of Cr(VI) resistance and reduction may differ from group to group; therefore, it is evident that both Cr(VI) resistance and reduction are shared abilities and not an exclusive characteristic of a single group, possibly reflecting horizontal genetic transfer resulting from selective pressure in this contaminated environment. Significance and Impact of the Study: To our knowledge, this is the first study of a microbial community under chronic chromate stress and, as the success of microbial-based metal remediation technologies requires a better understanding of the microbial community and the population response to metal stress, it may contribute to the implementation of a strategy of bioremediation of chromate-contaminated environments. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Diversity of chromium-resistant and -reducing bacteria in a chromium-contaminated activated sludge | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1046/j.1365-2672.2002.01591.x | en_US |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0002-4643-6075 | - |
crisitem.author.orcid | 0000-0001-7273-9371 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.