Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/8121
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Simões, Isaura | - |
dc.contributor.author | Mueller, Eva-Christina | - |
dc.contributor.author | Otto, Albrecht | - |
dc.contributor.author | Bur, Daniel | - |
dc.contributor.author | Cheung, Alice Y. | - |
dc.contributor.author | Faro, Carlos | - |
dc.contributor.author | Pires, Euclides | - |
dc.date.accessioned | 2009-02-09T11:10:47Z | - |
dc.date.available | 2009-02-09T11:10:47Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | FEBS Journal. 272:22 (2005) 5786-5798 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8121 | - |
dc.description.abstract | Cardosin A is an RGD-containing aspartic proteinase from the stigmatic papillae of Cynara cardunculus L. A putative cardosin A-binding protein has previously been isolated from pollen suggesting its potential involvement in pollen2013pistil interaction [Faro C, Ramalho-Santos M, Vieira M, Mendes A, Simões I, Andrade R, Verissimo P, Lin X, Tang J & Pires E (1999) J Biol Chem274, 28724201328729]. Here we report the identification of phospholipase D03B1 as a cardosin A-binding protein. The interaction was confirmed by coimmunoprecipitation studies and pull-down assays. To investigate the structural and molecular determinants involved in the interaction, pull-down assays with cardosin A and various glutathione S-transferase-fused phospholipase D03B1 constructs were performed. Results revealed that the C2 domain of phospholipase D03B1 contains the cardosin A-binding activity. Further assays with mutated recombinant forms of cardosin A showed that the RGD motif as well as the unprecedented KGE motif, which is structurally and charge-wise very similar to RGD, are indispensable for the interaction. Taken together our results indicate that the C2 domain of plant phospholipase D03B1 can act as a cardosin A-binding domain and suggest that plant C2 domains may have an additional role as RGD/KGE-recognition domains. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Molecular analysis of the interaction between cardosin A and phospholipase Dα | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1111/j.1742-4658.2005.04967.x | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-9331-6340 | - |
crisitem.author.orcid | 0000-0002-0480-8379 | - |
crisitem.author.orcid | 0000-0002-5853-0165 | - |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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