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https://hdl.handle.net/10316/8105
Title: | Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate | Authors: | Carvalho, Rui A. Babcock, Evelyn E. Jeffrey, F. Mark H. Sherry, A. Dean Malloy, Craig R. |
Issue Date: | 1999 | Citation: | Magnetic Resonance in Medicine. 42:1 (1999) 197-200 | Abstract: | Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc. | URI: | https://hdl.handle.net/10316/8105 | DOI: | 10.1002/(SICI)1522-2594(199907)42:1<197 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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