Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8099
Title: Quantitation of erythrocyte pentose pathway flux with [2-13C]glucose and 1H NMR analysis of the lactate methyl signal
Authors: Delgado, Teresa C. 
Castro, M. Margarida 
Geraldes, Carlos F. 
Jones, John G. 
Issue Date: 2004
Citation: Magnetic Resonance in Medicine. 51:6 (2004) 1283-1286
Abstract: A simple and sensitive NMR method for quantifying excess 13C-enrichment in positions 2 and 3 of lactate by 1H NMR spectroscopy of the lactate methyl signal is described. The measurement requires neither signal calibrations nor the addition of a standard and accounts for natural abundance 13C-contributions. As a demonstration, the measurement was applied to sim3 mumol of lactate generated by erythrocyte preparations incubated with [2-13C]glucose to determine the fraction of glucose metabolized by the pentose phosphate pathway (PP). PP fluxes were estimated from the ratio of excess 13C-enrichment in lactate carbon 3 relative to carbon 2 in accordance with established metabolic models. Under baseline conditions, PP flux accounted for 7 ± 2% of glucose consumption while in the presence of methylene blue, a classical activator of PP activity, its contribution increased to 27 ± 10% of total glucose consumption (P < 0.01). Magn Reson Med 51:1283-1286, 2004. © 2004 Wiley-Liss, Inc.
URI: http://hdl.handle.net/10316/8099
DOI: 10.1002/mrm.20096
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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