Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/7580
DC Field | Value | Language |
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dc.contributor.author | Monteiro, João | - |
dc.contributor.author | Videira, Romeu | - |
dc.contributor.author | Matos, Manuel | - |
dc.contributor.author | Dinis, Augusto | - |
dc.contributor.author | Jurado, Amália | - |
dc.date.accessioned | 2009-02-17T10:19:08Z | - |
dc.date.available | 2009-02-17T10:19:08Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Applied Biochemistry and Biotechnology. 150:3 (2008) 243-257 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/7580 | - |
dc.description.abstract | Abstract The Gram-positive bacterium, Bacillus stearothermophilus, was used as a model organism to identify the non-selective toxic effects of the currently used insecticide methoprene (isopropyl(2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate). A significant decrease of the yield of bacterial cultures and a premature appearance of ultrastructural abnormalities in cells cultured in the presence of the insecticide were taken as indicators of cytotoxicity. A putative correlation of this cytotoxicity with methoprene-induced perturbations on membrane lipid organization was investigated, using differential scanning calorimetry and the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and its propionic acid derivative (DPH–PA). The membrane physical effects depended on the lipid bilayer composition and packing. The most striking effect was a progressive broadening and shifting to lower temperatures, with increasing methoprene concentrations, of the main transition phase of the dimyristoyl- or dipalmitoylphosphatidylcholine bilayers and of the lateral phase separation of liposomes reconstituted with the lipid extracts of B. stearothermophilus. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Non-Selective Toxicological Effects of the Insect Juvenile Hormone Analogue Methoprene. A Membrane Biophysical Approach | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1007/s12010-007-8127-6 | en_US |
uc.controloAutoridade | Sim | - |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-4170-0092 | - |
crisitem.author.orcid | 0000-0001-7095-5337 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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