Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5455
Title: Adenosine A1 receptors inhibit Ca2+ channels coupled to the release of ACh, but not of GABA, in cultured retina cells
Authors: Santos, Paulo F. 
Caramelo, Olga L. 
Carvalho, Arsélio P. 
Duarte, Carlos B. 
Keywords: Acetylcholine; Release; Retina cell; Ca2+ imaging
Issue Date: 2000
Citation: Brain Research. 852:1 (2000) 10-15
Abstract: We investigated the effect of adenosine A1 receptors on the release of acetylcholine (ACh) and GABA, and on the intracellular calcium concentration ([Ca2+]i) response in cultured chick amacrine-like neurons, stimulated by KCl depolarization. The KCl-induced release of [3H]ACh, but not the release of [14C]GABA, was potentiated when adenosine A1 receptor activation was prevented by perfusing the cells with adenosine deaminase (ADA) or with 1,3-dipropyl-8-cycloentylxanthine (DPCPX). The changes in the [Ca2+]i induced by KCl depolarization, measured in neurite segments of single cultured cells, were also modulated by endogenous adenosine, acting on adenosine A1 receptors. Our results show that adenosine A1 receptors inhibit Ca2+ entry coupled to ACh release, but not to the release of GABA, suggesting that the synaptic vesicles containing each neurotransmitter are located in different zones of the neurites, containing different VSCC and/or different densities of adenosine A1 receptors.
URI: http://hdl.handle.net/10316/5455
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
file2e8c0c1588d44a6789b0c23d8b3e99cb.pdf135.57 kBAdobe PDFView/Open
Show full item record

Page view(s) 50

482
checked on Jun 3, 2020

Download(s)

84
checked on Jun 3, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.