Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5341
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dc.contributor.authorFernandes, Maria A. S.-
dc.contributor.authorCustódio, José B. A.-
dc.contributor.authorSantos, Maria S.-
dc.contributor.authorMoreno, António J. M.-
dc.contributor.authorVicente, Joaquim A. F.-
dc.date.accessioned2008-09-01T15:40:36Z-
dc.date.available2008-09-01T15:40:36Z-
dc.date.issued2006en_US
dc.identifier.citationMitochondrion. 6:4 (2006) 176-185en_US
dc.identifier.urihttp://hdl.handle.net/10316/5341-
dc.description.abstractThe effects of tetrandrine (6,6', 7,12-tetramethoxy-2, 2'-dimethyl-berbaman) on the mitochondrial function were assessed on oxidative stress, mitochondrial permeability transition (MPT), and bioenergetics of rat liver mitochondria. At concentrations lower than 100 nmol/mg protein, tetrandrine decreased the hydrogen peroxide formation, the extent of lipid peroxidation, the susceptibility to Ca2+-induced opening of MPT pore, and inhibited the inner membrane anion channel activity, not significantly affecting the mitochondrial bioenergetics. High tetrandrine concentrations (100-300 nmol/mg protein) stimulated succinate-dependent state 4 respiration, while some inhibition was observed for state 3 and p-trifluoromethoxyphenylhydrazone-uncoupled respirations. The respiratory control ratio and the transmembrane potential were depressed but the adenosine diphosphate to oxygen (ADP/O) ratio was less affected. A slight increase of the inner mitochondrial membrane permeability to H+ and K+ by tetrandrine was also observed. It was concluded that low concentrations of tetrandrine afford protection against liver mitochondria injury promoted by oxidative-stress events, such as hydrogen peroxide production, lipid peroxidation, and induction of MPT. Conversely, high tetrandrine concentrations revealed toxicological effects expressed by interference with mitochondrial bioenergetics, as a consequence of some inner membrane permeability to H+ and K+ and inhibition of the electron flux in the respiratory chain. The direct immediate protective role of tetrandrine against mitochondrial oxidative stress may be relevant to clarify the mechanisms responsible for its multiple pharmacological actions.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6W8G-4K8Y99X-1/1/34bae8ec6f49b23b674ee4e90404f49fen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBisbenzylisoquinoline alkaloideen_US
dc.subjectMitochondrial bioenergeticsen_US
dc.subjectPermeability transition poreen_US
dc.subjectTetrandrineen_US
dc.titleTetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stressen_US
dc.typearticleen_US
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.orcid0000-0002-6881-9392-
crisitem.author.orcid0000-0003-3575-7604-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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