Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5270
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Voss, T. | - |
dc.contributor.author | Gründler, P. | - |
dc.contributor.author | Brett, C. M. A. | - |
dc.contributor.author | Brett, A. M. Oliveira | - |
dc.date.accessioned | 2008-09-01T15:06:51Z | - |
dc.date.available | 2008-09-01T15:06:51Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Journal of Pharmaceutical and Biomedical Analysis. 19:1-2 (1999) 127-133 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5270 | - |
dc.description.abstract | The structural changes in cytochrome c with temperature have been been followed using a recently developed electrically-heated microelectrode sensor. Differential pulse voltammetry was used to perform electrochemical measurements of cytochrome c oxidation at different temperatures at heated bare gold electrodes contained in phosphate-buffered cytochrome c solution at room temperature. The voltammetric response shows the onset of unfolding and a marked dependence of the signal on electrode temperature. This augurs well for applications of heated electrodes as local probes in the study of the temperature dependence of electron transfer processes of other redox proteins, avoiding problems of bulk deterioration. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6TGX-3VKCCRN-D/1/171b8580ef00f2e0f90392182b57bde7 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Electrochemistry of cytochrome c | en_US |
dc.subject | Hot-wire electrodes | en_US |
dc.subject | Protein unfolding | en_US |
dc.title | Electrochemical behaviour of cytochrome c at electrically heated microelectrodes | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0731-7085(98)00189-7 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0002-1972-4434 | - |
crisitem.author.orcid | 0000-0002-6244-0891 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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filea7b0daa8155f4e108dd0c839642ba1c6.pdf | 165.68 kB | Adobe PDF | View/Open |
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