Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5133
DC Field | Value | Language |
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dc.contributor.author | Rebelo, Isabel A. | - |
dc.contributor.author | Piedade, José António P. | - |
dc.contributor.author | Brett, Ana Maria Oliveira | - |
dc.date.accessioned | 2008-09-01T15:04:31Z | - |
dc.date.available | 2008-09-01T15:04:31Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Talanta. 63:2 (2004) 323-331 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5133 | - |
dc.description.abstract | A selective method based on high performance liquid chromatography with electrochemical detection (HPLC-ECD) was developed to enable simultaneous detection of 8-oxo-7,8-dihydroguanine (8-oxoGua) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), products of DNA oxidative damage, in the presence of uric acid (UA), a strong interferent in their electrochemical detection. The method developed consists of HPLC isocratic elution with amperometric detection on a glassy carbon electrode, enabling a detection limit for 8-oxoGua and 8-oxodGuo lower than 1 nM in standard mixtures. Detection of low concentrations up to 25 nM of 8-oxoGua and 8-oxodGuo in the presence of UA in a 104-fold higher concentration was achieved after one-step solid phase extraction (SPE). The method was tested with urine samples and it was possible to detect and quantify the presence of 8-oxoGua, and to confirm that UA was eliminated after uricase degradation and SPE. The LOD found in urine samples was about 80 nM, a value higher than in standard mixtures, due to the increase of background current in the urine matrix. The results presented here contribute to the development of a methodological approach to simultaneous determination of 8-oxoGua and 8-oxodGuo in urine samples. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6THP-4BG45K9-1/1/203c0cf340998db82825488fecf36e47 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | DNA damage | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | 8-oxoGua | en_US |
dc.subject | 8-oxodGuo | en_US |
dc.subject | 8-oxo-7,8-Dihydroguanine | en_US |
dc.subject | 8-oxo-7,8-Dihydro-2'-deoxyguanosine | en_US |
dc.subject | Biomarker | en_US |
dc.subject | Uric acid | en_US |
dc.subject | HPLC-ECD | en_US |
dc.title | Development of an HPLC method with electrochemical detection of femtomoles of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine in the presence of uric acid | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.talanta.2003.10.051 | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0002-6244-0891 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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filebfd6ac1aa9664e19b3b39237359038ba.pdf | 165.58 kB | Adobe PDF | View/Open |
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