Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5080
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kramer-Marek, Gabriela | - |
dc.contributor.author | Serpa, Carlos | - |
dc.contributor.author | Szurko, Agnieszka | - |
dc.contributor.author | Widel, Maria | - |
dc.contributor.author | Sochanik, Aleksander | - |
dc.contributor.author | Snietura, Miroslaw | - |
dc.contributor.author | Kus, Piotr | - |
dc.contributor.author | Nunes, Rui M. D. | - |
dc.contributor.author | Arnaut, Luís G. | - |
dc.contributor.author | Ratuszna, Alicja | - |
dc.date.accessioned | 2008-09-01T15:03:36Z | - |
dc.date.available | 2008-09-01T15:03:36Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Journal of Photochemistry and Photobiology B: Biology. 84:1 (2006) 1-14 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5080 | - |
dc.description.abstract | Photodynamic therapy (PDT) and photodynamic diagnostics (PDD) of cancer are based on the use of non-toxic dyes (photosensitisers) in combination with harmless visible light. This paper reports physicochemical properties, cell uptake, localisation as well as photodynamic efficiency of two novel lipophilic porphyrin derivatives, suitable for use as PDT sensitisers. Both compounds are characterised by high quantum yield of singlet oxygen generation which was measured by time-resolved phosphorescence. Photodynamic in vitro studies were conducted on three cancer cell lines. Results of cell survival tests showed negligible dark cytotoxicity but high phototoxicity. The results also indicate that cell death is dependent on energy dose and time following light exposure. Using confocal laser scanning microscopy both compounds were found to localise in the cytoplasm around the nucleus of the tumour cells. The mode of cell death was evaluated based on the morphological changes after differential staining. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6TH0-4J9X306-1/1/a3dd4e8191b4f273644ef70f2a3cdbcc | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Photodynamic therapy | en_US |
dc.subject | Singlet oxygen | en_US |
dc.subject | Photosensitisation | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Necrosis | en_US |
dc.title | Spectroscopic properties and photodynamic effects of new lipophilic porphyrin derivatives: Efficacy, localisation and cell death pathways | en_US |
dc.type | article | en_US |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0001-7004-0110 | - |
crisitem.author.orcid | 0000-0002-3223-4819 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
file3fb912e70e6e4651837df17dd70f1736.pdf | 1.47 MB | Adobe PDF | View/Open |
Page view(s)
306
checked on Oct 15, 2024
Download(s) 50
529
checked on Oct 15, 2024
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.