Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/5045
Título: | An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes | Autor: | Ghica, Mariana Emilia Pauliukaite, Rasa Marchand, Nicolas Devic, Eric Brett, Christopher M. A. |
Palavras-chave: | Nicotinamide adenine dinucleotide oxidase; Aldehyde dehydrogenase; Carbon film electrode; Poly(neutral red); Enzyme cross-linking; Sol-gel | Data: | 2007 | Citação: | Analytica Chimica Acta. 591:1 (2007) 80-86 | Resumo: | Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine. | URI: | https://hdl.handle.net/10316/5045 | DOI: | 10.1016/j.aca.2007.03.047 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Química - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
file4f15aa88007b4da096c2460d69a1adb2.pdf | 559.26 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
56
Visto em 19/ago/2024
Citações WEB OF SCIENCETM
52
Visto em 2/fev/2024
Visualizações de página
320
Visto em 27/ago/2024
Downloads
348
Visto em 27/ago/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.