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http://hdl.handle.net/10316/5045
Title: | An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes | Authors: | Ghica, Mariana Emilia Pauliukaite, Rasa Marchand, Nicolas Devic, Eric Brett, Christopher M.A. |
Keywords: | Nicotinamide adenine dinucleotide oxidase; Aldehyde dehydrogenase; Carbon film electrode; Poly(neutral red); Enzyme cross-linking; Sol-gel | Issue Date: | 2007 | Citation: | Analytica Chimica Acta. 591:1 (2007) 80-86 | Abstract: | Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine. | URI: | http://hdl.handle.net/10316/5045 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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