Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5045
Title: An improved biosensor for acetaldehyde determination using a bienzymatic strategy at poly(neutral red) modified carbon film electrodes
Authors: Ghica, Mariana Emilia 
Pauliukaite, Rasa 
Marchand, Nicolas 
Devic, Eric 
Brett, Christopher M.A. 
Keywords: Nicotinamide adenine dinucleotide oxidase; Aldehyde dehydrogenase; Carbon film electrode; Poly(neutral red); Enzyme cross-linking; Sol-gel
Issue Date: 2007
Citation: Analytica Chimica Acta. 591:1 (2007) 80-86
Abstract: Improved biosensors for acetaldehyde determination have been developed using a bienzymatic strategy, based on a mediator-modified carbon film electrode and co-immobilisation of NADH oxidase and aldehyde dehydrogenase. Modification of the carbon film electrode with poly(neutral red) mediator resulted in a sensitive, low-cost and reliable NADH detector. Immobilisation of the enzymes was performed using encapsulation in a sol-gel matrix or cross-linking with glutaraldehyde. The bienzymatic biosensors were characterized by studying the influence of pH, applied potential and co-factors. The sol-gel and glutaraldehyde biosensors showed a linear response up to 60 [mu]M and 100 [mu]M, respectively, with detection limits of 2.6 [mu]M and 3.3 [mu]M and sensitivities were 1.7 [mu]A mM-1 and 5.6 [mu]A mM-1. The optimised biosensors showed good stability and good selectivity and have been tested for application for the determination of acetaldehyde in natural samples such as wine.
URI: http://hdl.handle.net/10316/5045
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
file4f15aa88007b4da096c2460d69a1adb2.pdf559.26 kBAdobe PDFView/Open
Show full item record

Page view(s)

147
checked on Dec 10, 2019

Download(s)

65
checked on Dec 10, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.