Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/4852
DC Field | Value | Language |
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dc.contributor.author | Rego, Ana Cristina | - |
dc.contributor.author | Sancha Santos, Maria | - |
dc.contributor.author | Oliveira, Catarina Resende | - |
dc.date.accessioned | 2008-09-01T14:15:34Z | - |
dc.date.available | 2008-09-01T14:15:34Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Neurochemistry International. 36:2 (1999) 159-166 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/4852 | - |
dc.description.abstract | Glutamate is an excitotoxin responsible for causing neuronal damage associated with mitochondria dysfunction. We have analyzed the relationship between the mitochondrial respiratory rate, the membrane potential ([Delta][Psi]) and the activity of mitochondrial complexes in retinal cells in culture, used as neuronal models. Glutamate (10 [mu]M-10 mM) dose-dependently decreased the O2 consumption and the membrane potential. A linear correlation was found between these parameters, suggesting that the mitochondrial respiratory function was affected. Exposure to glutamate (100 [mu]M) for 10 min, in the absence of Mg2+, inhibited the activity of complex I (26.3%), complexes II/III (22.2%) and complex IV (26.7%). MK-801 ((+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine hydrogen maleate), a non-competitive antagonist of the NMDA (N-methyl--aspartate) receptors, completely reversed the effect exerted by 100 [mu]M glutamate at the level of complexes I, II/III and IV. These results suggest that NMDA receptor-mediated inhibition of mitochondrial respiratory chain complexes may be responsible for the alteration in the respiratory rate of chick retinal cells submitted to glutamate. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T0B-43MK6KX-9/1/6efac4a526cb12bdb79436e4c10b9294 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Glutamate | en_US |
dc.subject | Mitochondria | en_US |
dc.subject | NMDA receptor | en_US |
dc.subject | Retinal cells | en_US |
dc.title | Glutamate-mediated inhibition of oxidative phosphorylation in cultured retinal cells | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0197-0186(99)00107-2 | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-0700-3776 | - |
crisitem.author.orcid | 0000-0002-6881-9392 | - |
crisitem.author.orcid | 0000-0001-6942-4328 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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filebdb1e503d23840419030920bdfcff834.pdf | 203.75 kB | Adobe PDF | View/Open |
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