Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/4845
DC Field | Value | Language |
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dc.contributor.author | Ambrósio, António F. | - |
dc.contributor.author | Silva, Ana P. | - |
dc.contributor.author | Malva, João O. | - |
dc.contributor.author | Soares-da-Silva, Patrício | - |
dc.contributor.author | Carvalho, Arsélio P. | - |
dc.contributor.author | Carvalho, Caetana M. | - |
dc.date.accessioned | 2008-09-01T14:15:27Z | - |
dc.date.available | 2008-09-01T14:15:27Z | - |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Neuropharmacology. 38:9 (1999) 1349-1359 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/4845 | - |
dc.description.abstract | In order to better understand the mechanism(s) of action of carbamazepine (CBZ), we studied its effects on the increase in [Ca2+]i and [Na+]i stimulated by glutamate ionotropic receptor agonists, in cultured rat hippocampal neurons, as followed by indo-1 or SBFI fluorescence, respectively. CBZ inhibited the increase in [Ca2+]i stimulated either by glutamate, kainate, [alpha]-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA), or N-methyl--aspartate (NMDA), in a concentration-dependent manner. In order to discriminate the effects of CBZ on the activation of glutamate receptors from possible effects on Ca2+ channels, we determined the inhibitory effects of Ca2+ channel blockers on [Ca2+]i changes in the absence or in the presence of CBZ. The presence of 1 [mu]M nitrendipine, 0.5 [mu]M [omega]-conotoxin GVIA ([omega]-CgTx GVIA), or of both blockers, inhibited the kainate-stimulated increase in [Ca2+]i by 51.6, 32.9 or 68.7%, respectively. In the presence of both 100 [mu]M CBZ and nitrendipine, the inhibition was similar (54.1%) to that obtained with nitrendipine alone, but in the presence of both CBZ and [omega]-CgTx GVIA, the inhibition was greater (54%) than that caused by [omega]-CgTx GVIA alone. However, CBZ did not inhibit the increase in [Na+]i stimulated by the glutamate receptor agonists, but inhibited the increase in [Na+]i due to veratridine. Tetrodotoxin, or MK-801, did not inhibit the influx of Na+ stimulated by kainate, indicating that Na+ influx occurs mainly through the glutamate ionotropic non-NMDA receptors. Moreover, LY 303070, a specific AMPA receptor antagonist, inhibited the [Na+]i response to kainate or AMPA by about 70 or 80%, respectively, suggesting that AMPA receptors are mainly involved. Taken together, the results suggest that CBZ inhibits L-type Ca2+ channels and Na+ channels, but does not inhibit activation of glutamate ionotropic receptors. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T0C-3X1W5Y4-D/1/65c32835840a01052a606b944eb4cabd | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Carbamazepine | en_US |
dc.subject | Glutamate ionotropic receptors | en_US |
dc.subject | Kainate | en_US |
dc.subject | Voltage-sensitive Ca2+ channels | en_US |
dc.subject | Voltage-sensitive Na+ channels | en_US |
dc.subject | [Ca2+]i | en_US |
dc.title | Carbamazepine inhibits L-type Ca2+ channels in cultured rat hippocampal neurons stimulated with glutamate receptor agonists | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0028-3908(99)00058-1 | - |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0477-1641 | - |
crisitem.author.orcid | 0000-0002-5438-4447 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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file0a433b4aa20944869326bfa62c3195c1.pdf | 194.42 kB | Adobe PDF | View/Open |
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