Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/4797
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gil, Joana | - |
dc.contributor.author | Almeida, Sandra | - |
dc.contributor.author | Oliveira, Catarina R. | - |
dc.contributor.author | Rego, A. Cristina | - |
dc.date.accessioned | 2008-09-01T14:14:38Z | - |
dc.date.available | 2008-09-01T14:14:38Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Free Radical Biology and Medicine. 35:11 (2003) 1500-1514 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/4797 | - |
dc.description.abstract | In this study, we investigated the involvement of reactive oxygen species (ROS) and calcium in staurosporine (STS)-induced apoptosis in cultured retinal neurons, under conditions of maintained membrane integrity. The antioxidants idebenone (IDB), glutathione-ethylester (GSH/EE), trolox, and Mn(III)tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) significantly reduced STS-induced caspase-3-like activity and intracellular ROS generation. Endogenous sources of ROS production were investigated by testing the effect of the following inhibitors: 7-nitroindazole (7-NI), a specific inhibitor of the neuronal isoform of nitric oxide synthase (nNOS); arachidonyl trifluoromethyl ketone (AACOCF3), a phospholipase A2 (PLA2) inhibitor; allopurinol, a xanthine oxidase inhibitor; and the mitochondrial inhibitors rotenone and oligomycin. All these compounds decreased caspase-3-like activity and ROS generation, showing that both mitochondrial and cytosolic sources of ROS are implicated in this mechanism. STS induced a significant increase in intracellular calcium concentration ([Ca2+]i), which was partially prevented in the presence of IDB and GSH/EE, indicating its dependence on ROS generation. These two antioxidants and the inhibitors allopurinol and 7-NI also reduced the number of TdT-mediated dUTP nick-end labeling-positive cells. Thus, endogenous ROS generation and the rise in intracellular calcium are important inter-players in STS-triggered apoptosis. Furthermore, the antioxidants may help to prolong retinal cell survival upon apoptotic cell death. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T38-4B1Y892-D/1/de8345fb5df912dddbc572b28cc1f4be | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Apoptosis | en_US |
dc.subject | Antioxidants | en_US |
dc.subject | Calcium | en_US |
dc.subject | Caspase-3 | en_US |
dc.subject | Mitochondria | en_US |
dc.subject | Reactive oxygen species | en_US |
dc.subject | Retinal cells | en_US |
dc.subject | Staurosporine | en_US |
dc.subject | Free radicals | en_US |
dc.title | Cytosolic and mitochondrial ROS in staurosporine-induced retinal cell apoptosis | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/j.freeradbiomed.2003.08.022 | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-6942-4328 | - |
crisitem.author.orcid | 0000-0003-0700-3776 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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filee631db6392ac4c68a1af41f6eab39b3e.pdf | 396.6 kB | Adobe PDF | View/Open |
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