Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/4790
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cunha, Rodrigo A. | - |
dc.contributor.author | Ribeiro, J. A. | - |
dc.contributor.author | Malva, João O. | - |
dc.date.accessioned | 2008-09-01T14:14:31Z | - |
dc.date.available | 2008-09-01T14:14:31Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Neurochemistry International. 44:5 (2004) 371-379 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/4790 | - |
dc.description.abstract | Kainate receptors are ionotropic glutamate receptors located postsynaptically, mediating frequency-dependent transmission, and presynaptically, modulating transmitter release. In contrast to the excitatory postsynaptic kainate receptors, presynaptic kainate receptor can also be inhibitory and their effects may involve a metabotropic action. Arachidonic acid (AA) modulates most ionotropic receptors, in particular postsynaptic kainate receptor-mediated currents. To further explore differences between pre- and postsynaptic kainate receptors, we tested if presynaptic kainate receptors are affected by AA. Kainate (0.3-3 [mu]M) and the kainate receptor agonist, domoate (60-300 nM), inhibited by 19-54% the field excitatory postsynaptic potential (fEPSP) slope in rat CA1 hippocampus, and increased by 12-32% paired-pulse facilitation (PPF). AA (10 [mu]M) attenuated by 37-72% and by 62-66% the domoate (60-300 nM)-induced fEPSP inhibition and paired-pulse facilitation increase, respectively. This inhibition by AA was unaffected by cyclo- and lipo-oxygenase inhibitors, indomethacin (20 [mu]M) and nordihydroguaiaretic acid (NDGA, 50 [mu]M) or by the free radical scavenger, N-acetyl--cysteine (0.5 mM). The K+ (20 mM)-evoked release of [3H]glutamate from superfused hippocampal synaptosomes was inhibited by 18-39% by domoate (1-10 [mu]M), an effect attenuated by 35-63% by AA (10 [mu]M). Finally, the KD (40-55 nM) of the kainate receptor agonist [3H]-(2S,4R)-4-methylglutamate ([3H]MGA) (0.3-120 nM) binding to hippocampal synaptosomal membranes was increased by 151-329% by AA (1-10 [mu]M). These results indicate that AA directly inhibits presynaptic kainate receptor controlling glutamate release in the CA1 area of the rat hippocampus. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6T0B-49JHH14-1/1/306d6b018d3a5d33b6a0973186ea4ed9 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Kainate | en_US |
dc.subject | Arachidonic acid | en_US |
dc.subject | Hippocampus | en_US |
dc.subject | Synaptic transmission | en_US |
dc.subject | Nerve terminals | en_US |
dc.subject | Glutamate release | en_US |
dc.subject | Binding | en_US |
dc.title | Presynaptic kainate receptors modulating glutamatergic transmission in the rat hippocampus are inhibited by arachidonic acid | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S0197-0186(03)00167-0 | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-2550-6422 | - |
crisitem.author.orcid | 0000-0002-5438-4447 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
file311cfd16de6f4d41a62150aae249ff28.pdf | 174.37 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
9
checked on Oct 7, 2024
WEB OF SCIENCETM
Citations
7
checked on Oct 2, 2024
Page view(s) 50
590
checked on Oct 8, 2024
Download(s)
362
checked on Oct 8, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.