Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/4776
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dc.contributor.authorAlmeida, Sandra-
dc.contributor.authorDomingues, António-
dc.contributor.authorRodrigues, Luís-
dc.contributor.authorOliveira, Catarina R.-
dc.contributor.authorRego, A. Cristina-
dc.date.accessioned2008-09-01T14:14:16Z-
dc.date.available2008-09-01T14:14:16Z-
dc.date.issued2004en_US
dc.identifier.citationNeurobiology of Disease. 17:3 (2004) 435-444en_US
dc.identifier.urihttp://hdl.handle.net/10316/4776-
dc.description.abstractThe mitochondrial toxin 3-nitropropionic acid (3-NP) has been largely used to study neurodegenerative disorders in which bioenergetic defects are implicated. In the present study, we analyzed the molecular pathways involved in FK506 neuroprotection against cell death induced by 3-NP, using cultured cortical neurons. 3-NP induced cytochrome c release and increased caspases -2, -3, -8, and -9-like activities, although, calpain activity was not significantly affected. FK506 decreased cytochrome c release and caspase-3-like activity induced by 3-NP, without changing the activities of other caspases. FK-506 also decreased the number of apoptotic neurons, determined by Hoechst. Under these conditions, FK506 alone significantly reduced calcineurin activity by about 50%. Our results also showed a decrease in mitochondrial Bax and an increase in mitochondrial Bcl-2 levels upon exposure to FK506 and 3-NP. However, no significant changes occurred in total Bcl-2 and Bax levels. Altogether, the results suggest that FK506 neuroprotection against 3-NP-induced apoptosis is associated with the redistribution of Bcl-2 and Bax in the mitochondrial membrane.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WNK-4DFBTRN-1/1/437ab5ac7896585944bdb87bca46a539en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subject3-Nitropropionic aciden_US
dc.subjectMitochondriaen_US
dc.subjectApoptosisen_US
dc.subjectCortical neuronsen_US
dc.titleFK506 prevents mitochondrial-dependent apoptotic cell death induced by 3-nitropropionic acid in rat primary cortical culturesen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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