Please use this identifier to cite or link to this item:
Title: The DNA extraction from the pulp dentine complex of both with and without carious
Authors: Corte-Real, A. 
Andrade, L. 
Anjos, M. J. 
Carvalho, M. 
Vide, M. C. 
Corte-Real, F. 
Vieira, D. N. 
Keywords: Pulp-dentine-complex; Forensic DNA
Issue Date: 2006
Citation: International Congress Series. 1288:(2006) 710-712
Abstract: The main objective of this paper is to show that, despite some adverse forensic condition such as degraded human body remains and exhumed material, the dentine (in pulp dentine complex) keeps, in the majority of cases, its integrity. In this study, we use a sample of 30 human teeth (both with and without carious) after extraction during dental treatment. We analyze 15 STRs and both high-variable regions I and II of mitochondrial DNA. Each tooth was prepared using a technique that comprises the mechanic removal of the enamel, central pulp and cement. The DNA extraction was carried out with a commercial kit, but the protocol was adjusted according to the specificities of the sample. This procedure has allowed us to obtain a genetic profile of mitochondria DNA in all the samples as well as to define a profile of STRs in some of them.
DOI: 10.1016/j.ics.2005.11.053
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
filecd714c89fe4449a3b536f3c15d823c90.pdf63.12 kBAdobe PDFView/Open
Show full item record


checked on Nov 9, 2022

Page view(s) 50

checked on Nov 28, 2022


checked on Nov 28, 2022

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.