Please use this identifier to cite or link to this item:
Title: Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice
Authors: Codices, Vera 
Martins, Catarina 
Novo, Carlos 
Pinho, Mário 
Sousa, Bruno 
Lopes, Ângela 
Borrego, Miguel 
Matos, Olga 
Keywords: Animals; Cryptosporidiosis; Cryptosporidium parvum; Eosinophils; Female; Immunity, Cellular; Immunocompetence; Immunocompromised Host; Lymphocytes; Mice; Mice, Inbred BALB C; Monocytes; Neutrophils; Spleen; T-Lymphocyte Subsets
Issue Date: 2013
Serial title, monograph or event: Acta Parasitologica
Volume: 58
Issue: 1
Abstract: Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22. Fecal and blood samples, spleens, and small intestines were collected for analysis. Peripheral blood and spleen cell populations were characterized by flow cytometry. After infection (days 0 to 21), mice presented higher values of neutrophils, eosinophils, NK cells and CD4(+)CD25(high) T cells in peripheral blood. After reinfection, this upward trend continued in the following days for all four populations in infected mice. At day 35, infected mice presented similar values to the control group, except for CD4(+)CD25(high) T cells, which remained higher in infected mice. A possible correlation between alterations in blood and spleen cell populations was also studied, but no consistent association could be established. Small intestine sections were screened for intracellular stages of the parasite but no evidence of pathology was observed. Here, we report information which may be important for the understanding of the specific cell-mediated response in immunocompetent mice to C. parvum infection. Although some questions remain unanswered and complementary studies are needed, our results are expected to contribute to a better understanding of innate and Treg cells role in the clearance process of this parasite.
DOI: 10.2478/s11686-013-0113-2
Rights: openAccess
Appears in Collections:I&D CINEICC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
2013_Codices_et_al.pdf633.11 kBAdobe PDFView/Open
Show full item record


checked on May 29, 2020


checked on May 29, 2020

Page view(s)

checked on Aug 5, 2020


checked on Aug 5, 2020

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.