Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/3894
DC Field | Value | Language |
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dc.contributor.author | Salgado, António P. | - |
dc.contributor.author | Santos, Rosa M. | - |
dc.contributor.author | Fernandes, Ana P. | - |
dc.contributor.author | Tomé, Ângelo R. | - |
dc.contributor.author | Flatt, Peter R. | - |
dc.contributor.author | Rosário, Luís M. | - |
dc.date.accessioned | 2008-08-29T15:35:45Z | - |
dc.date.available | 2008-08-29T15:35:45Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | The International Journal of Biochemistry & Cell Biology. 32:5 (2000) 557-569 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/3894 | - |
dc.description.abstract | Using clonal insulin-secreting BRIN-BD11 cells, we have assessed whether the graded response of the whole cell population to glucose can be accounted for by a dose-dependent recruitment of individual cells, an amplification of the response of the recruited cells or both. Cytosolic free Ca2+ concentration ([Ca2+]i) is an established index of [beta]-cell function. We used fura-2 microfluorescence techniques to assess the [Ca2+]i responsiveness of single BRIN-BD11 cells to glucose and other secretagogues. Glucose (1-16.7Â mM) evoked oscillatory [Ca2+]i rises in these cells resembling those found in parental rat pancreatic [beta]-cells. The percentage of glucose-responsive cells was 11% at 1Â mM and increased to 40-70% at 3-16.7Â mM glucose, as assessed by a single-stimulation protocol. This profile was unrelated to possible differences in the cell cycle, as inferred from experiments where the cultured cells were synchronized by a double thymidine block protocol. Individual cells exhibited variable sensitivities to glucose (threshold range: 1-5Â mM) and a variable dose-dependent amplification of the [Ca2+]i responses (EC50 range: 2-10Â mM), as assessed by a multiple-stimulation protocol. Glyceraldehyde and [alpha]-ketoisocaproic acid had glucose-like effects on [Ca2+]i. The data support a mixed model for the activation of insulin-secreting cells. Specifically, the graded secretory response of the whole cell population is likely to reflect both a recruitment of individual cells with different sensitivities to glucose and a dose-dependent amplification of the response of the recruited cells. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6TCH-3YW26B7-B/1/53849b978f1051f5cac6464bd7012ee8 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Pancreatic [beta]-cell line | en_US |
dc.subject | Glucose metabolism | en_US |
dc.subject | Ca2+ oscillations | en_US |
dc.subject | Fura-2 fluorescence | en_US |
dc.subject | Cellular heterogeneity | en_US |
dc.title | Glucose-mediated Ca2+ signalling in single clonal insulin-secreting cells: evidence for a mixed model of cellular activation | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1016/S1357-2725(99)00146-6 | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-0789-8637 | - |
crisitem.author.orcid | 0000-0001-8671-989X | - |
crisitem.author.orcid | 0000-0001-8329-2333 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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