Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/3871
Title: Effects of Li+ transport and Li+ immobilization on Li+/Mg2+ competition in cells: implications for bipolar disorder
Authors: Layden, Brian T. 
Abukhdeir, Abde M. 
Williams, Nicole 
Fonseca, Carla P. 
Carroll, Laura 
Castro, Margarita M. C. A. 
Geraldes, Carlos F. G. C. 
Bryant, Fred B. 
Freitas, Duarte Mota de 
Keywords: Fluorescence; Atomic absorption; Neuroblastoma; Lymphoblastoma
Issue Date: 2003
Citation: Biochemical Pharmacology. 66:10 (2003) 1915-1924
Abstract: Li+/Mg2+ competition has been implicated in the therapeutic action of Li+ treatment in bipolar illness. We hypothesized that this competition depended on cell-specific properties. To test this hypothesis, we determined the degree of Li+ transport, immobilization, and Li+/Mg2+ competition in lymphoblastomas, neuroblastomas, and erythrocytes. During a 50 mmol/L Li+-loading incubation, Li+ accumulation at 30 min (mmoles Li+/L cells) was the greatest in lymphoblastomas (11.1±0.3), followed by neuroblastomas (9.3±0.5), and then erythrocytes (4.0±0.5). Li+ binding affinities to the plasma membrane in all three cell types were of the same order of magnitude; however, Li+ immobilization in intact cells was greatest in neuroblastomas and least in erythrocytes. When cells were loaded for 30 min in a 50 mmol/L Li+-containing medium, the percentage increase in free intracellular [Mg2+] in neuroblastoma and lymphoblastoma cells (~55 and ~52%, respectively) was similar, but erythrocytes did not exhibit any substantial increase (~6%). With the intracellular [Li+] at 15 mmol/L, the free intracellular [Mg2+] increased by the greatest amount in neuroblastomas (~158%), followed by lymphoblastomas (~75%), and then erythrocytes (~50%). We conclude that Li+ immobilization and transport are related to free intracellular [Mg2+] and to the extent of Li+/Mg2+ competition in a cell-specific manner.
URI: http://hdl.handle.net/10316/3871
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
filef8947563fb734aa8bd890120d23d6590.pdf230.56 kBAdobe PDFView/Open
Show full item record

Page view(s)

82
checked on Sep 11, 2019

Download(s)

57
checked on Sep 11, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.