Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/23325
Title: Mitochondrial complex I dysfunction induced by cocaine and cocaine plus morphine in brain and liver mitochondria
Authors: Cunha-Oliveira, Teresa 
Silva, Lisbeth 
Silva, Ana Maria 
Moreno, António J. 
Oliveira, Catarina R. 
Santos, Maria S. 
Keywords: speedball; mitochondria
Issue Date: 1-May-2013
Serial title, monograph or event: Toxicology Letters
Volume: 219
Abstract: Mitochondrial function and energy metabolism are affected in brains of human cocaine abusers. Cocaine is known to induce mitochondrial dysfunction in cardiac and hepatic tissues, but its effects on brain bioenergetics are less documented. Furthermore, the combination of cocaine and opioids (speedball) was also shown to induce mitochondrial dysfunction. In this work, we compared the effects of cocaine and/or morphine on the bioenergetics of isolated brain and liver mitochondria, to understand their specific effects in each tissue. Upon energization with complex I substrates, cocaine decreased state-3 respiration in brain (but not in liver) mitochondria and decreased uncoupled respiration and mitochondrial potential in both tissues, through a direct effect on complex I. Morphine presented only slight effects on brain and liver mitochondria, and the combination cocaine+morphine had similar effects to cocaine alone, except for a greater decrease in state-3 respiration. Brain and liver mitochondrial respirations were differentially affected, and liver mitochondria were more prone to proton leak caused by the drugs or their combination. This was possibly related with a different dependence on complex I in mitochondrial populations from these tissues. In summary, cocaine and cocaine+morphine induce mitochondrial complex I dysfunction in isolated brain and liver mitochondria, with specific effects in each tissue.
URI: http://hdl.handle.net/10316/23325
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
cunha-oliveiraetaltoxicologyletters.pdf1.62 MBAdobe PDFView/Open
Show full item record

Page view(s) 20

576
checked on Jul 15, 2020

Download(s) 20

751
checked on Jul 15, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.