Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/12831
Title: Activation of neuropeptide Y receptors is neuroprotective against excitotoxicity in organotypic hippocampal slice cultures
Authors: Silva, Ana P. 
Pinheiro, Paulo S. 
Carvalho, Arsélio P. 
Carvalho, Caetana M. 
Jakobsen, Birthe 
Zimmer, Jens 
Malva, João O. 
Keywords: NPY; AMPA; Kainate; Neuroprotection; Neurodegeneration
Issue Date: Jun-2003
Publisher: The Federation of American Societies for Experimental Biology
Citation: The FASEB Journal. 17:9 (2003) 1118-1120
Abstract: Glutamate and NPY have been implicated in hippocampal neuropathology in temporal lobe epilepsy. Thus, we investigated the involvement of NPY receptors in mediating neuroprotection against excitotoxic insults in organotypic cultures of rat hippocampal slices. Exposure of hippocampal slice cultures to 2 μM AMPA (α-amino-3-hydroxy-5-methyl-isoxazole-4- propionate) induced neuronal degeneration, monitored by propidium iodide uptake, of granule cells and CA1 pyramidal cells. For dentate granule cells, selective activation of Y1, Y2, or Y5 receptors with 1 μM [Leu31,Pro34]NPY, 300 nM NPY13–36 or 1 μM 500 nM NPY(19–23)- 1 3 4 6 31 32 34 (Gly ,Ser ,Gln ,Thr ,Ala ,Aib ,Gln )-PP, respectively, had a neuroprotective effect against AMPA, whereas only the activation of Y2 receptors was effective for CA1 pyramidal cells. When the slice cultures were exposed to 6 μM kainate, the CA3 pyramidal cells displayed significant degeneration, and in this case the activation of Y1, Y2, and Y5 receptors was neuroprotective. For the kainic acid-induced degeneration of CA1 pyramidal cells, it was again found that only the Y2 receptor activation was effective. Based on the present findings, it was concluded that Y1, Y2, and Y5 receptors effectively can modify glutamate receptor-mediated neurodegeneration in the hippocampus
URI: http://hdl.handle.net/10316/12831
ISSN: 1530-6860
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Activation of neuropeptide Y receptors is neuroprotective.pdf2.47 MBAdobe PDFView/Open
Show full item record

Page view(s)

145
checked on Jul 30, 2020

Download(s)

109
checked on Jul 30, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.