Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/12624
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dc.contributor.authorBernardino, Liliana-
dc.contributor.authorXapelli, Sara-
dc.contributor.authorSilva, Ana P.-
dc.contributor.authorJakobsen, Birthe-
dc.contributor.authorPoulsen, Frantz R.-
dc.contributor.authorOliveira, Catarina R.-
dc.contributor.authorVezzani, Annamaria-
dc.contributor.authorMalva, João O.-
dc.contributor.authorZimmer, Jens-
dc.date.accessioned2010-03-01T12:52:07Z-
dc.date.available2010-03-01T12:52:07Z-
dc.date.issued2005-07-20-
dc.identifier.citationThe Journal of Neuroscience. 25:29 (2005) 6734-6744en_US
dc.identifier.issn1529-2401-
dc.identifier.urihttp://hdl.handle.net/10316/12624-
dc.description.abstractThe inflammatory cytokines interleukin-1\u2424 and tumor necrosis factor-\u2423 (TNF-\u2423) have been identified as mediators of several forms of neurodegeneration in the brain. However, they can produce either deleterious or beneficial effects on neuronal function. We investigated the effects of these cytokines on neuronal death caused by exposure of mouse organotypic hippocampal slice cultures to toxic concen- trations of AMPA. Either potentiation of excitotoxicity or neuroprotection was observed, depending on the concentration of the cytokines and the timing of exposure. A relatively high concentration of mouse recombinant TNF-\u2423 (10 ng/ml) enhanced excitotoxicity when the cultures were simultaneously exposed to AMPA and to this cytokine. Decreasing the concentration of TNF-\u2423 to 1 ng/ml resulted in neuroprotection against AMPA-induced neuronal death independently on the application protocol. By using TNF-\u2423 receptor (TNFR) knock-out mice, we demonstrated that the potentiation of AMPA-induced toxicity by TNF-\u2423 involves TNF receptor-1, whereas the neuroprotective effect is mediated by TNF receptor-2. AMPA exposure was associated with activation and proliferation of microglia as assessed by macrophage antigen-1 and bromodeoxyuridine immunohistochemistry, suggesting a functional recruitment of cytokine- producing cells at sites of neurodegeneration. Together, these findings are relevant for understanding the role of proinflammatory cytokines and microglia activation in acute and chronic excitotoxic conditionsen_US
dc.language.isoengen_US
dc.publisherSociety for Neuroscienceen_US
dc.rightsopenAccessen_US
dc.subjectGlutamateen_US
dc.subjectCytokinesen_US
dc.subjectInflammationen_US
dc.subjectNeuroprotectionen_US
dc.subjectNeurodegenerationen_US
dc.subjectMicrogliaen_US
dc.titleModulator effects of interleukin-1beta and tumor necrosis factor-alpha on AMPA-induced excitotoxicity in mouse organotypic hippocampal slice culturesen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0002-5438-4447-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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