Please use this identifier to cite or link to this item:
Title: Screening of Five Essential Oils for Identification of Potential Inhibitors of IL-1-induced Nf-κB Activation and NO Production in Human Chondrocytes: Characterization of the Inhibitory Activity of α-Pinene
Authors: Neves, Ângela 
Rosa, Susana 
Gonçalves, Juliana 
Rufino, Ana 
Judas, Fernando 
Salgueiro, Lígia 
Lopes, Celeste 
Cavaleiro, Carlos 
Mendes, Alexandrina Ferreira 
Keywords: Arthritis; α-Pinene; IL-1β; Essential oil; NF-κB; Nitric Oxide
Issue Date: 2009
Publisher: Thieme Publishers
Citation: Planta Med. 75 (2009)
Abstract: Nuclear factor-κB is a key transcription factor activated by pro-inflammatory signals, like interleukin-1β (IL-1), being required for the expression of many inflammatory and catabolic mediators, such as nitric oxide (NO), that play an important role in arthritic diseases. This work aimed at screening and identifying natural inhibitors of IL-induced NF-κB activation and NO production in human articular chondrocytes. Five essential oils obtained from four plants of the Iberian flora, Mentha x piperita L. (Lamiaceae), Origanum virens L. (Lamiaceae), Lavandula luiseri L. (Lamiaceae), and Juniperus oxycedrus L. subsp. oxycedrus (Cupressaceae), were screened for their ability to prevent IL-1-induced NO production. The oil showing higher inhibitory activity was fractionated, concentrated, analyzed for composition elucidation and prepared for further assays. For this purpose, the human chondrocytic cell line C-28/I2 was used to evaluate NF-κB activation by determining the cytoplasmic levels of the total and phosphorylated forms of the inhibitory protein, IκB-α, and the NF- κB‑DNAbinding activity. The essential oil from the leaves of J. oxycedrus in a concentration of 0.02% (v/v) achieved the greatest inhibition (80 ± 8%) of IL-1-induced NO production. Chemical analysis showed that this essential oil is predominantly composed of monoterpene hydrocabons, being α-pinene [2,6,6-trimethyl-bicyclo(3.1.1)hept-3- ene] the major constituent (76%). Similarly to the effect of the whole oil, a fraction containing 93% α- pinene reduced significantly IL-1-induced IκB-α degradation. Moreover, α-pinene also decreased IκB-α phosphorylation, NF-κB‑DNA binding activity, and NO production. Another fraction containing oxygenated mono- and sesquiterpenes was nearly as effective as α-pinene. The ability of the α-pinene-containing fraction to reduce IL-1-induced NF-κB activation and NO production warrants further studies to demonstrate the usefulness of α-pinene in the treatment of arthritic diseases and other conditions in which NF-κB and NO play pathological roles.
DOI: 10.1055/s-0029-1186085
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
ManuscriptAccepted_PlantaMed.pdf197.55 kBAdobe PDFView/Open
Show full item record


checked on Nov 17, 2022


checked on May 2, 2023

Page view(s) 50

checked on Apr 17, 2024

Download(s) 20

checked on Apr 17, 2024

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.