Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/116151
DC Field | Value | Language |
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dc.contributor.author | Martins-Marques, Tânia | - |
dc.contributor.author | Costa, Marina C | - |
dc.contributor.author | Catarino, Steve | - |
dc.contributor.author | Simões, Isaura | - |
dc.contributor.author | Aasen, Trond | - |
dc.contributor.author | Enguita, Francisco J | - |
dc.contributor.author | Girao, Henrique | - |
dc.date.accessioned | 2024-08-21T14:04:08Z | - |
dc.date.available | 2024-08-21T14:04:08Z | - |
dc.date.issued | 2022-07-05 | - |
dc.identifier.issn | 1469-3178 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/116151 | - |
dc.description.abstract | Through the exchange of lipids, proteins, and nucleic acids, extracellular vesicles (EV) allow for cell-cell communication across distant cells and tissues to regulate a wide range of physiological and pathological processes. Although some molecular mediators have been discovered, the mechanisms underlying the selective sorting of miRNAs into EV remain elusive. Previous studies demonstrated that connexin43 (Cx43) forms functional channels at the EV surface, mediating the communication with recipient cells. Here, we show that Cx43 participates in the selective sorting of miRNAs into EV through a process that can also involve RNA-binding proteins. We provide evidence that Cx43 can directly bind to specific miRNAs, namely those containing stable secondary structure elements, including miR-133b. Furthermore, Cx43 facilitates the delivery of EV-miRNAs into recipient cells. Phenotypically, we show that Cx43-mediated EV-miRNAs sorting modulates autophagy. Overall, our study ascribes another biological role to Cx43, that is, the selective incorporation of miRNAs into EV, which potentially modulates multiple biological processes in target cells and may have implications for human health and disease. | pt |
dc.description.sponsorship | This work was supported by the European Regional Development Fund (ERDF) through the Operational Program for Competitiveness Factors (COMPETE) (under the projects HealthyAging2020 CENTRO-01-0145-FEDER-000012- N2323; CENTRO-01-0145-FEDER-032179, CENTRO-01-0145-FEDER-032414, POCI-01-0145-FEDER-022122, UIDB/04539/2020 and UIDP/04539/2020), COST Action EU-CARDIOPROTECTION, supported by COST (European Cooperation in Science and Technology), Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) under grant agreement No 952266 (Project RESETageing) and PPBI-Portuguese Platform of BioImaging: POCI-01-0145-FEDER-022122. TA acknowledges funding from Instituto de Salud Carlos III grant PI21/00470 co-financed by the European Regional Development Fund (ERDF), and Fundacion Cientıfica Asociacion Espanola Contra el Cancer (IDEAS SEMILLA AECC 2020/IDEAS20039AASE). | pt |
dc.language.iso | eng | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP/04539/2020 | pt |
dc.rights | openAccess | pt |
dc.subject | Connexin43; heterogeneous nuclear ribonucleoproteins; intercellular communication; miRNA; selective sorting | pt |
dc.subject.mesh | Cell Communication | pt |
dc.subject.mesh | Cell Movement | pt |
dc.subject.mesh | Connexin 43 | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Extracellular Vesicles | pt |
dc.subject.mesh | MicroRNAs | pt |
dc.title | Cx43-mediated sorting of miRNAs into extracellular vesicles | pt |
dc.type | article | - |
degois.publication.firstPage | e54312 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | EMBO reports | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.15252/embr.202154312 | pt |
degois.publication.volume | 23 | pt |
dc.date.embargo | 2022-07-05 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-5798-3923 | - |
crisitem.author.orcid | 0000-0001-9644-6960 | - |
crisitem.author.orcid | 0000-0002-9331-6340 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Cx43-mediated sorting of miRNAs into extracellular vesicles.pdf | 2.3 MB | Adobe PDF | View/Open |
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