The assessment of hepatotoxic effect of bioactive compounds derived from citrullus colocynthis organic extracts

Abstract

The use of traditional medicines is increasing day by day. As, the herbal products are more preferable than the synthetic medicines. The current study was designed to evaluate potential of bioactive compounds through in-vivo and in-vitro methodology; in the aforesaid study16 rats were randomly divided into four groups (3 experimental and 1 controls) to check the cytotoxicity of C. colocynthis. In the experimental groups a single daily dose of Methanolic, n-hexane and chloroform extract of C. colocynthis (300, 600, 900, 1200 mg/kg) was administered orally. Normal saline was administered in control group. After 15 days, the rats were sacrificed under deep anaesthesia and liver histopathology was done. The results indicated the minimal morphological changes of liver cells even by maximum dose of 1200mg/kg. However, the biochemical parameters like ALP, AST, and ALT can confirm that the toxicity effects are dose dependent. C. colocynthis have Phytoconstituents (Gallic Acid, Vanillic Acid, Feralic Acid, Cinamic acid, Ether, 3-butenyl pentyl, oxime-, methoxy-phenyl-_ Ethyl 4 chlorobutanimidoate) which were confirmed by HPLC and GC-MS. These phytochemicals have remarkable anti-oxidant, anti-diabetic, thrombolytic and haemolytic activities.