Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114029
DC Field | Value | Language |
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dc.contributor.author | Traldi, Federico | - |
dc.contributor.author | Liu, Pengfei | - |
dc.contributor.author | Albino, Inês | - |
dc.contributor.author | Ferreira, Lino | - |
dc.contributor.author | Zarbakhsh, Ali | - |
dc.contributor.author | Resmini, Marina | - |
dc.date.accessioned | 2024-03-15T11:10:52Z | - |
dc.date.available | 2024-03-15T11:10:52Z | - |
dc.date.issued | 2023-02-01 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/114029 | - |
dc.description.abstract | Biomedical applications of nanoparticles require a fundamental understanding of their interactions and behavior with biological interfaces. Protein corona formation can alter the morphology and properties of nanomaterials, and knowledge of the interfacial behavior of the complexes, using in situ analytical techniques, will impact the development of nanocarriers to maximize uptake and permeability at cellular interfaces. In this study we evaluate the interactions of acrylamide-based nanogels, with neutral, positive, and negative charges, with serum-abundant proteins albumin, fibrinogen, and immunoglobulin G. The formation of a protein corona complex between positively charged nanoparticles and albumin is characterized by dynamic light scattering, circular dichroism, and surface tensiometry; we use neutron reflectometry to resolve the complex structure at the air/water interface and demonstrate the effect of increased protein concentration on the interface. Surface tensiometry data suggest that the structure of the proteins can impact the interfacial properties of the complex formed. These results contribute to the understanding of the factors that influence the bio-nano interface, which will help to design nanomaterials with improved properties for applications in drug delivery. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 764958 (FT, IA). The authors wish to thank the Institut Laue-Langevin (ILL), France for neutron beamtime on the FIGARO reflectometer (DOI: 10.5291/ILL-DATA.9-12-588 and 10.5291/ILL-DATA.9-13-838). We would also like to thank Armando Maestro for the assistance with NR measurements. The Chinese Scholarship Council (CSC) funding is gratefully acknowledged for sponsoring P.L. (PhD studentship, 201606150067). LF also would like to acknowledge the funding of the FCT project with the acronym ENDEAVOUR (Ref: EXPL/BTMORG/ 1348/2021) and PT2020 project with the acronym BIO-MED (Ref: 181228). IA was supported by FCT (Ref: 2021.07471.BD). | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | surface tensiometry | pt |
dc.subject | protein corona | pt |
dc.subject | nanogels | pt |
dc.subject | interfacial behavior | pt |
dc.subject | neutron reflectivity | pt |
dc.subject.mesh | Nanogels | pt |
dc.subject.mesh | Water | pt |
dc.subject.mesh | Polymers | pt |
dc.subject.mesh | Albumins | pt |
dc.subject.mesh | Surface Properties | pt |
dc.subject.mesh | Protein Corona | pt |
dc.subject.mesh | Nanoparticles | pt |
dc.title | Protein-Nanoparticle Interactions Govern the Interfacial Behavior of Polymeric Nanogels: Study of Protein Corona Formation at the Air/Water Interface | pt |
dc.type | article | - |
degois.publication.firstPage | 2810 | pt |
degois.publication.issue | 3 | pt |
degois.publication.title | International Journal of Molecular Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms24032810 | pt |
degois.publication.volume | 24 | pt |
dc.date.embargo | 2023-02-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-8985-9302 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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