Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/114011
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | González-Riquelme, María José | - |
dc.contributor.author | Lucas-Ruiz, Fernando | - |
dc.contributor.author | Galindo-Romero, Caridad | - |
dc.contributor.author | Boia, Raquel | - |
dc.contributor.author | Ambrósio, António Francisco | - |
dc.contributor.author | Vidal-Sanz, Manuel | - |
dc.contributor.author | Santiago, Ana Raquel | - |
dc.contributor.author | Agudo-Barriuso, Marta | - |
dc.date.accessioned | 2024-03-14T11:32:39Z | - |
dc.date.available | 2024-03-14T11:32:39Z | - |
dc.date.issued | 2023-02-09 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/114011 | - |
dc.description.abstract | Retinal organotypic cultures (ROCs) are used as an in vivo surrogate to study retinal ganglion cell (RGC) loss and neuroprotection. In vivo, the gold standard to study RGC degeneration and neuroprotection is optic nerve lesion. We propose here to compare the course of RGC death and glial activation between both models. The left optic nerve of C57BL/6 male mice was crushed, and retinas analyzed from 1 to 9 days after the injury. ROCs were analyzed at the same time points. As a control, intact retinas were used. Retinas were studied anatomically to assess RGC survival, microglial, and macroglial activation. Macroglial and microglial cells showed different morphological activation between models and were activated earlier in ROCs. Furthermore, microglial cell density in the ganglion cell layer was always lower in ROCs than in vivo. RGC loss after axotomy and in vitro followed the same trend up to 5 days. Thereafter, there was an abrupt decrease in viable RGCs in ROCs. However, RGC somas were still immuno-identified by several molecular markers. ROCs are useful for proof-of-concept studies on neuroprotection, but long-term experiments should be carried out in vivo. Importantly, the differential glial activation observed between models and the concomitant death of photoreceptors that occurs in vitro may alter the efficacy of RGC neuroprotective therapies when tested in in vivo models of optic nerve injury. | pt |
dc.description.sponsorship | PID2019-106498GB-I00; PI19/00071; 19881/GERM/15 | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | CENTRO-01-0145-FEDER-000008 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDB/04539/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | microglia | pt |
dc.subject | Müller cells | pt |
dc.subject | retinal ganglion cells | pt |
dc.subject | astrocytes | pt |
dc.subject | axotomy | pt |
dc.subject | in vivo | pt |
dc.subject | in vitro | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Male | pt |
dc.subject.mesh | Mice, Inbred C57BL | pt |
dc.subject.mesh | Retina | pt |
dc.subject.mesh | Retinal Ganglion Cells | pt |
dc.subject.mesh | Axotomy | pt |
dc.subject.mesh | Cell Survival | pt |
dc.subject.mesh | Microphysiological Systems | pt |
dc.subject.mesh | Optic Nerve Injuries | pt |
dc.title | Comparative Analysis of Retinal Organotypic Cultures and In Vivo Axotomized Retinas | pt |
dc.type | article | - |
degois.publication.firstPage | 3481 | pt |
degois.publication.issue | 4 | pt |
degois.publication.title | International Journal of Molecular Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms24043481 | pt |
degois.publication.volume | 24 | pt |
dc.date.embargo | 2023-02-09 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-0477-1641 | - |
crisitem.author.orcid | 0000-0002-7541-7041 | - |
Aparece nas coleções: | I&D CIBB - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Comparative-Analysis-of-Retinal-Organotypic-Cultures-and-In-Vivo-Axotomized-RetinasInternational-Journal-of-Molecular-Sciences.pdf | 8.94 MB | Adobe PDF | Ver/Abrir |
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