Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/113492
DC Field | Value | Language |
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dc.contributor.author | Cordeiro, Daniela | - |
dc.contributor.author | Camelo, Alexandra | - |
dc.contributor.author | Pedrosa, Ana Carolina | - |
dc.contributor.author | Brandão, Inês | - |
dc.contributor.author | Canhoto, Jorge | - |
dc.contributor.author | Espirito Santo, Christophe | - |
dc.contributor.author | Correia, Sandra | - |
dc.date.accessioned | 2024-02-21T12:24:58Z | - |
dc.date.available | 2024-02-21T12:24:58Z | - |
dc.date.issued | 2023-03-15 | - |
dc.identifier.issn | 2409-9279 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/113492 | - |
dc.description.abstract | Long-read sequencing methods allow a comprehensive analysis of transcriptomes in identifying full-length transcripts. This revolutionary method represents a considerable breakthrough for non-model species since it allows enhanced gene annotation and gene expression studies when compared to former sequencing methods. However, woody plant tissues are challenging to the successful preparation of cDNA libraries, thus, impairing further cutting-edge sequencing analyses. Here, a detailed protocol for preparing cDNA libraries suitable for high throughput RNA sequencing using Oxford Nanopore Technologies® is described. This method was used to prepare eight barcoded cDNA libraries from two Solanum betaceum cell lines: one with compact morphology and embryogenic competency (EC) and another with friable and non-embryogenic (NEC). The libraries were successfully sequenced, and data quality assessment showed high mean quality scores. Using this method, long-read sequencing will allow a comprehensive analysis of plant transcriptomes. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | The Foundation for Science and Technology (Portugal) supported Daniela Cordeiro’s PhD fellowship (SFRH/BD/136925/2018). This work was carried out at the R&D Unit Center for Functional Ecology—Science for People and the Planet (UIDB/04004/2020), financed by FCT/MCTES through national funds (PIDDAC), and at CATAA, this work was made in the scope of CULTIVAR project (CENTRO-01-0145-FEDER-000020), co-financed by the Regional Operational Programme Centro 2020, Portugal 2020, and the European Union, through the European Fund for Regional Development (ERDF), Collaborative Project. | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | amplification-free protocol | pt |
dc.subject | direct cDNA sequencing | pt |
dc.subject | high-throughput sequencing | pt |
dc.subject | MinION | pt |
dc.subject | Oxford Nanopore Technologies® | pt |
dc.subject | poly(A) RNA | pt |
dc.subject | Solanaceae | pt |
dc.subject | Solanum betaceum | pt |
dc.subject | transcriptome | pt |
dc.subject | woody plant | pt |
dc.title | An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Long-Read Sequencing | pt |
dc.type | article | - |
degois.publication.firstPage | 31 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | Methods and Protocols | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/mps6020031 | pt |
degois.publication.volume | 6 | pt |
dc.date.embargo | 2023-03-15 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.researchunit | CFE - Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.orcid | 0000-0003-2299-298X | - |
crisitem.author.orcid | 0000-0002-9800-4186 | - |
crisitem.author.orcid | 0000-0003-2151-3916 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CFE - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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An-Efficient-Method-to-Prepare-Barcoded-cDNA-Libraries-from-Plant-Callus-for-LongRead-SequencingMethods-and-Protocols.pdf | 17.28 MB | Adobe PDF | View/Open |
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