Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/113139
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jorge, Joana | - |
dc.contributor.author | Neves, Joana | - |
dc.contributor.author | Alves, Raquel | - |
dc.contributor.author | Geraldes, Catarina | - |
dc.contributor.author | Gonçalves, Ana Cristina | - |
dc.contributor.author | Sarmento-Ribeiro, Ana Bela | - |
dc.date.accessioned | 2024-02-06T13:07:38Z | - |
dc.date.available | 2024-02-06T13:07:38Z | - |
dc.date.issued | 2023-05-23 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/113139 | - |
dc.description.abstract | Lymphoid malignancies are a group of highly heterogeneous diseases frequently associated with constitutive activation of the nuclear factor kappa B (NF-κB) signaling pathway. Parthenolide is a natural compound used to treat migraines and arthritis and found to act as a potent NF-κB signaling inhibitor. This study evaluated in vitro parthenolide efficacy in lymphoid neoplasms. We assessed parthenolide metabolic activity in NCI-H929 (MM), Farage (GCB-DLBCL), Raji (BL), 697 and KOPN-8 (B-ALL), and CEM and MOLT-4 (T-ALL), by resazurin assay. Cell death, cell cycle, mitochondrial membrane potential (ΔΨmit), reactive oxygen species (ROS) and reduced glutathione (GSH) levels, activated caspase-3, FAS-ligand, and phosphorylated NF-κB p65 were evaluated using flow cytometry. CMYC, TP53, GPX1, and TXRND1 expression levels were assessed using qPCR. Our results showed that parthenolide promoted a metabolic activity decrease in all cell lines in a time-, dose-, and cell-line-dependent manner. The mechanism induced by parthenolide was demonstrated to be cell line dependent. Nonetheless, parthenolide promoted cell death by apoptosis with significant ROS increase (peroxides and superoxide anion) and GSH decrease combined with a ΔΨmit reduction across all studied cell lines. Despite the need to further understand parthenolide mechanisms, parthenolide should be considered as a possible new therapeutic approach for B- and T-lymphoid malignancies. | pt |
dc.description.sponsorship | The present work was supported by CIMAGO—Center of Investigation on Environment, Genetics and Oncobiology, Faculty of Medicine, University of Coimbra, Portugal (Project 18/12), by the Foundation for Science and Technology (FCT), Portugal (Strategic Projects UID/NEU/04539/2013 and UID/NEU/04539/2019) and COMPETE-FEDER (POCI-01-0145-FEDER-007440). FCT supported Joana Jorge with a Ph.D. grant (SFRH/BD/145531/2019). | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2019 | pt |
dc.relation | SFRH/BD/145531/2019 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | acute lymphoblastic leukemia | pt |
dc.subject | multiple myeloma | pt |
dc.subject | lymphoma | pt |
dc.subject | NF- B | pt |
dc.subject | NF- B inhibitors | pt |
dc.subject | apoptosis | pt |
dc.subject | oxidative stress | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | NF-kappa B | pt |
dc.subject.mesh | Reactive Oxygen Species | pt |
dc.subject.mesh | Cell Line, Tumor | pt |
dc.subject.mesh | Apoptosis | pt |
dc.subject.mesh | I-kappa B Proteins | pt |
dc.subject.mesh | Sesquiterpenes | pt |
dc.subject.mesh | Lymphoma | pt |
dc.title | Parthenolide Induces ROS-Mediated Apoptosis in Lymphoid Malignancies | pt |
dc.type | article | - |
degois.publication.firstPage | 9167 | pt |
degois.publication.issue | 11 | pt |
degois.publication.title | International Journal of Molecular Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms24119167 | pt |
degois.publication.volume | 24 | pt |
dc.date.embargo | 2023-05-23 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | CNC. IBILI | - |
crisitem.project.grantno | CNC. IBILI | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-2599-6433 | - |
crisitem.author.orcid | 0000-0003-1470-4802 | - |
crisitem.author.orcid | 0000-0002-4142-4841 | - |
Appears in Collections: | I&D CIBB - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Parthenolide-Induces-ROSMediated-Apoptosis-in-Lymphoid-MalignanciesInternational-Journal-of-Molecular-Sciences.pdf | 2.85 MB | Adobe PDF | View/Open |
Page view(s)
109
checked on Oct 16, 2024
Download(s)
37
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License