Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/111927
Title: | Lipidome of extracellular vesicles from Giardia lamblia | Authors: | Faria, Clarissa Perez Ferreira, Bárbara Lourenço, Ágata Guerra, Inês Calado Melo, Tânia Domingues, Pedro Domingues, Maria do Rosário Marques Cruz, Maria Teresa Sousa, Maria do Céu |
Issue Date: | 2023 | Publisher: | Public Library of Science | Project: | PTDC/SAU-PAR/31506/2017 UIDB/04539/2020 UIDP/50017/2020 UIDB/50017/2020 LA/P/0094/2020 UIDB/50006/2020 LISBOA-01-0145-FEDER-402-022125 2021.04754.BD CEECIND/ 01578/2020 |
Serial title, monograph or event: | PLoS ONE | Volume: | 18 | Issue: | 9 | Abstract: | Extracellular vesicles (EVs) (exossomes, microvesicles and apoptotic bodies) have been well acknowledged as mediators of intercellular communications in prokaryotes and eukaryotes. Lipids are essential molecular components of EVs but at the moment the knowledge about the lipid composition and the function of lipids in EVs is limited and as for now none lipidomic studies in Giardia EVs was described. Therefore, the focus of the current study was to conduct, for the first time, the characterization of the polar lipidome, namely phospholipid and sphingolipid profiles of G. lamblia trophozoites, microvesicles (MVs) and exosomes, using C18-Liquid Chromatography-Mass Spectrometry (C18-LC-MS) and Tandem Mass Spectrometry (MS/MS). A total of 162 lipid species were identified and semi-quantified, in the trophozoites, or in the MVs and exosomes belonging to 8 lipid classes, including the phospholipid classes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), cardiolipins (CL), the sphingolipid classes sphingomyelin (SM) and ceramides (Cer), and cholesterol (ST), and 3 lipid subclasses that include lyso PC (LPC), lyso PE (LPE) and lyso PG (LPG), but showing different abundances. This work also identified, for the first time, in G. lamblia trophozoites, the lipid classes CL, Cer and ST and subclasses of LPC, LPE and LPG. Univariate and multivariate analysis showed clear discrimination of lipid profiles between trophozoite, exosomes and MVs. The principal component analysis (PCA) plot of the lipidomics dataset showed clear discrimination between the three groups. Future studies focused on the composition and functional properties of Giardia EVs may prove crucial to understand the role of lipids in host-parasite communication, and to identify new targets that could be exploited to develop novel classes of drugs to treat giardiasis. | URI: | https://hdl.handle.net/10316/111927 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0291292 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Lipidome-of-extracellular-vesicles-from-Giardia-lambliaPLoS-ONE.pdf | 1.86 MB | Adobe PDF | View/Open |
Page view(s)
75
checked on Oct 16, 2024
Download(s)
46
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License