Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/111616
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Maranha, Ana | - |
dc.contributor.author | Costa, Mafalda | - |
dc.contributor.author | Ripoll-Rozada, Jorge | - |
dc.contributor.author | Manso, José A. | - |
dc.contributor.author | Miranda, Vanessa | - |
dc.contributor.author | Mendes, Vera M. | - |
dc.contributor.author | Manadas, Bruno | - |
dc.contributor.author | Macedo-Ribeiro, Sandra | - |
dc.contributor.author | Ventura, M. Rita | - |
dc.contributor.author | Pereira, Pedro José Barbosa | - |
dc.contributor.author | Empadinhas, Nuno | - |
dc.date.accessioned | 2024-01-08T17:41:15Z | - |
dc.date.available | 2024-01-08T17:41:15Z | - |
dc.date.issued | 2023-01-27 | - |
dc.identifier.issn | 2399-3642 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/111616 | - |
dc.description.abstract | The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) so far detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyltransferase implicated the adjacent genes in MMP biosynthesis. We report a wide distribution of this gene cluster in NTM, including slowly growing mycobacteria such as Mycobacterium avium, which we reveal to produce MMP. Using a combination of MMP purification and chemoenzymatic syntheses of intermediates, we identified the biosynthetic mechanism of MMP, relying on two enzymes that we characterized biochemically and structurally: a previously undescribed α-endomannosidase that hydrolyses MMP into defined-sized mannoligosaccharides that prime the elongation of new daughter MMP chains by a rare α-(1→4)-mannosyltransferase. Therefore, MMP biogenesis occurs through a partially conservative replication mechanism, whose disruption affected mycobacterial growth rate at low temperature. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Nature | pt |
dc.relation | PTDC/BIA-MIC/0122/2021 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDP/04539/2020 | pt |
dc.relation | LA/P/0058/2020 | pt |
dc.relation | SFRH/BD/101191/2014 | pt |
dc.relation | DL57/2016/CP1355/CT0011 | pt |
dc.relation | SFRH/BPD/108004/2015 | pt |
dc.relation | LISBOA-01-0145-FEDER-007660 | pt |
dc.relation | PINFRA/22125/2016 | pt |
dc.relation | PTDC/BTM-TEC/29221/2017 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Lipopolysaccharides | pt |
dc.subject.mesh | Mannosyltransferases | pt |
dc.subject.mesh | Methyltransferases | pt |
dc.subject.mesh | Mycobacterium | pt |
dc.title | Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides | pt |
dc.type | article | - |
degois.publication.firstPage | 108 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Communications Biology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s42003-023-04448-3 | pt |
degois.publication.volume | 6 | pt |
dc.date.embargo | 2023-01-27 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - Associate Laboratory | - |
crisitem.project.grantno | RNEM Portuguese Mass Spectrometry Network | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-9005-8377 | - |
crisitem.author.orcid | 0000-0003-2023-5491 | - |
crisitem.author.orcid | 0000-0002-4593-673X | - |
crisitem.author.orcid | 0000-0002-2087-4042 | - |
crisitem.author.orcid | 0000-0001-8938-7560 | - |
Appears in Collections: | IIIUC - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Selfrecycling-and-partially-conservative-replication-of-mycobacterial-methylmannose-polysaccharidesCommunications-Biology.pdf | 5.71 MB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License