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Title: | Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene | Authors: | Matos, Teresa J. Jaleco, Sara P. Gonçalo, Margarida Duarte, Carlos B. Lopes, M. Celeste |
Issue Date: | 14-Aug-2005 | Publisher: | Hindawi | Project: | FCT and FEDER | Serial title, monograph or event: | Mediators of Inflammation | Volume: | 2005 | Issue: | 3 | Abstract: | We used a mouse fetal skin dendritic cell line (FSDC) to study the effect of the strong allergen 2,4-dinitrofluorobenzene (DNFB) on interleukin (IL)-1beta release and IL-1beta receptor immunoreactivity. Stimulation with DNFB (30 minutes) increased IL-1 release without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin-1beta-converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z-Tyr-Val-Ala-Asp-AFC. The ICE inhibitor Z-YVAD-FMK prevented the release of IL-1beta evoked by DNFB. Incubation of the cells with DNFB (30 minutes) strongly increased IL-1beta receptor immunoreactivity. The rapid effect of DNFB on the release of mature IL-1beta, without inducing an increase of IL-1beta mRNA in FSDC, suggests a posttranslational modification of pro-IL-1beta by ICE activity. | URI: | https://hdl.handle.net/10316/110948 | ISSN: | 0962-9351 | DOI: | 10.1155/MI.2005.131 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais |
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Release-of-IL1-via-IL1converting-enzyme-in-a-skin-dendritic-cell-line-exposed-to-24dinitrofluorobenzeneMediators-of-Inflammation.pdf | 1.69 MB | Adobe PDF | View/Open |
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