Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/110053
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dc.contributor.authorSousa-Ferreira, Lígia-
dc.contributor.authorGarrido, Manuel Joaquim Marques-
dc.contributor.authorNascimento-Ferreira, Isabel-
dc.contributor.authorNóbrega, Clévio-
dc.contributor.authorCarvalho, Ana dos Santos-
dc.contributor.authorÁlvaro, Ana Rita Costa Silva-
dc.contributor.authorSalgado, Joana Rosmaninho-
dc.contributor.authorKaster, Manuella-
dc.contributor.authorKügler, Sebastian-
dc.contributor.authorAlmeida, Luís Pereira de-
dc.contributor.authorCavadas, Cláudia-
dc.date.accessioned2023-11-14T12:06:37Z-
dc.date.available2023-11-14T12:06:37Z-
dc.date.issued2011-
dc.identifier.issn1932-6203pt
dc.identifier.urihttps://hdl.handle.net/10316/110053-
dc.description.abstractNeuropeptide Y (NPY) produced by arcuate nucleus (ARC) neurons has a strong orexigenic effect on target neurons. Hypothalamic NPY levels undergo wide-ranging oscillations during the circadian cycle and in response to fasting and peripheral hormones (from 0.25 to 10-fold change). The aim of the present study was to evaluate the impact of a moderate long-term modulation of NPY within the ARC neurons on food consumption, body weight gain and hypothalamic neuropeptides. We achieved a physiological overexpression (3.6-fold increase) and down-regulation (0.5-fold decrease) of NPY in the rat ARC by injection of AAV vectors expressing NPY and synthetic microRNA that target the NPY, respectively. Our work shows that a moderate overexpression of NPY was sufficient to induce diurnal over-feeding, sustained body weight gain and severe obesity in adult rats. Additionally, the circulating levels of leptin were elevated but the immunoreactivity (ir) of ARC neuropeptides was not in accordance (POMC-ir was unchanged and AGRP-ir increased), suggesting a disruption in the ability of ARC neurons to response to peripheral metabolic alterations. Furthermore, a dysfunction in adipocytes phenotype was observed in these obese rats. In addition, moderate down-regulation of NPY did not affect basal feeding or normal body weight gain but the response to food deprivation was compromised since fasting-induced hyperphagia was inhibited and fasting-induced decrease in locomotor activity was absent.These results highlight the importance of the physiological ARC NPY levels oscillations on feeding regulation, fasting response and body weight preservation, and are important for the design of therapeutic interventions for obesity that include the NPY.pt
dc.language.isoengpt
dc.publisherPublic Library of Sciencept
dc.relationThis work was supported by the Portuguese Foundation for Science and Technology, FEDER and COMPETE [PTDC/SAU-FCF/099082/2008; PTDC/SAUFCF/ 70384/2006; PTDC/SAU/NEU/73119/2006; SFRH/BD/30608/2006; SFRH/BD/29479/2006; SFRH/BPD/62945/2009; SFRH/BD/45311/2008, SFRH/BPD/49079/2008; SFRH/BPD/46086/2008; SFRH/BPD/31547/2006]pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshAdipocytespt
dc.subject.meshAdipogenesispt
dc.subject.meshAnimalspt
dc.subject.meshArcuate Nucleus of Hypothalamuspt
dc.subject.meshCell Sizept
dc.subject.meshDependoviruspt
dc.subject.meshDown-Regulationpt
dc.subject.meshEatingpt
dc.subject.meshFastingpt
dc.subject.meshFeedback, Physiologicalpt
dc.subject.meshGenetic Vectorspt
dc.subject.meshHEK293 Cellspt
dc.subject.meshHumanspt
dc.subject.meshHyperphagiapt
dc.subject.meshMalept
dc.subject.meshMicroRNAspt
dc.subject.meshNeuronspt
dc.subject.meshNeuropeptide Ypt
dc.subject.meshObesitypt
dc.subject.meshPPAR gammapt
dc.subject.meshRatspt
dc.subject.meshRats, Wistarpt
dc.subject.meshTime Factorspt
dc.subject.meshWeight Gainpt
dc.subject.meshEnergy Metabolismpt
dc.titleModerate long-term modulation of neuropeptide Y in hypothalamic arcuate nucleus induces energy balance alterations in adult ratspt
dc.typearticle-
degois.publication.firstPagee22333pt
degois.publication.issue7pt
degois.publication.titlePLoS ONEpt
dc.peerreviewedyespt
dc.identifier.doi10.1371/journal.pone.0022333pt
degois.publication.volume6pt
dc.date.embargo2011-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
crisitem.author.deptInstitute of Interdisciplinary Research-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCIBB - Center for Innovative Biomedicine and Biotechnology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCIBB - Center for Innovative Biomedicine and Biotechnology-
crisitem.author.orcid0000-0003-0421-0681-
crisitem.author.orcid0000-0002-8312-5292-
crisitem.author.orcid0000-0003-4718-5666-
crisitem.author.orcid0000-0002-6781-8515-
crisitem.author.orcid0000-0001-5831-3307-
crisitem.author.orcid0000-0001-8020-9266-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
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