Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/110007
Title: | FRET in Membrane Biophysics: An Overview | Authors: | Loura, Luís M. S. Prieto, Manuel |
Keywords: | energy transfer; fluorescence; lipid bilayers; lipid–proteininteraction; lipid rafts | Issue Date: | 2011 | Publisher: | Frontiers Media S.A. | Project: | FCOMP- 01-0124-FEDER-010787(FCTPTDC/QUI-QUI/098198/2008 PTDC/QUI-BIQ/099947/2008 PTDC/QUI-BIQ/112067 |
Serial title, monograph or event: | Frontiers in Physiology | Volume: | 2 | Issue: | NOV | Abstract: | Förster resonance energy transfer (FRET), in most applications used as a "spectroscopic ruler," allows an easy determination of the donor-acceptor intermolecular distance. However, the situation becomes complex in membranes, since around each donor there is an ensemble of acceptors at non-correlated distances. In this review, state-of-the-art methodologies for this situation are presented, usually involving time-resolved data and model fitting. This powerful approach can be used to study the occurrence of phase separation ("rafts" or other type of domains), allowing their detection as well as size evaluation. Formalisms for studying lipid-protein and protein-protein interactions according to specific topologies are also addressed. The advantages and added complexity of a specific type of FRET (energy homotransfer or energy migration) are described, as well as applications of FRET under the microscope. | URI: | https://hdl.handle.net/10316/110007 | ISSN: | 1664042X | DOI: | 10.3389/fphys.2011.00082 | Rights: | openAccess |
Appears in Collections: | I&D CQC - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FRET in membrane biophysics An overview.pdf | 613.61 kB | Adobe PDF | View/Open |
Page view(s)
58
checked on Oct 16, 2024
Download(s)
21
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License