Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/109774
DC Field | Value | Language |
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dc.contributor.author | Xapelli, Sara Alves | - |
dc.contributor.author | Agasse, Fabienne | - |
dc.contributor.author | Sardà-Arroyo, Laura | - |
dc.contributor.author | Bernardino, Liliana | - |
dc.contributor.author | Santos, Tiago | - |
dc.contributor.author | Ribeiro, Filipa F. | - |
dc.contributor.author | Valero, Jorge | - |
dc.contributor.author | Bragança, José | - |
dc.contributor.author | Schitine, Clarissa | - |
dc.contributor.author | de Melo Reis, Ricardo A | - |
dc.contributor.author | Sebastião, Ana M. | - |
dc.contributor.author | Malva, João O. | - |
dc.date.accessioned | 2023-10-26T08:57:37Z | - |
dc.date.available | 2023-10-26T08:57:37Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1932-6203 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/109774 | - |
dc.description.abstract | The endocannabinoid system has been implicated in the modulation of adult neurogenesis. Here, we describe the effect of type 1 cannabinoid receptor (CB1R) activation on self-renewal, proliferation and neuronal differentiation in mouse neonatal subventricular zone (SVZ) stem/progenitor cell cultures. Expression of CB1R was detected in SVZ-derived immature cells (Nestin-positive), neurons and astrocytes. Stimulation of the CB1R by (R)-(+)-Methanandamide (R-m-AEA) increased self-renewal of SVZ cells, as assessed by counting the number of secondary neurospheres and the number of Sox2+/+ cell pairs, an effect blocked by Notch pathway inhibition. Moreover, R-m-AEA treatment for 48 h, increased proliferation as assessed by BrdU incorporation assay, an effect mediated by activation of MAPK-ERK and AKT pathways. Surprisingly, stimulation of CB1R by R-m-AEA also promoted neuronal differentiation (without affecting glial differentiation), at 7 days, as shown by counting the number of NeuN-positive neurons in the cultures. Moreover, by monitoring intracellular calcium concentrations ([Ca(2+)]i) in single cells following KCl and histamine stimuli, a method that allows the functional evaluation of neuronal differentiation, we observed an increase in neuronal-like cells. This proneurogenic effect was blocked when SVZ cells were co-incubated with R-m-AEA and the CB1R antagonist AM 251, for 7 days, thus indicating that this effect involves CB1R activation. In accordance with an effect on neuronal differentiation and maturation, R-m-AEA also increased neurite growth, as evaluated by quantifying and measuring the number of MAP2-positive processes. Taken together, these results demonstrate that CB1R activation induces proliferation, self-renewal and neuronal differentiation from mouse neonatal SVZ cell cultures. | pt |
dc.language.iso | eng | pt |
dc.publisher | Public Library of Science | pt |
dc.relation | POCTI/SAU-NEU/68465/2006 | pt |
dc.relation | PTDC/SAU-NEU/ 104415/2008 | pt |
dc.relation | PTDC/SAU-NEU/101783/2008 | pt |
dc.relation | POCTI/SAU-NEU/110838/2009 | pt |
dc.relation | Fundação Calouste Gulbenkian (project nu 96542) | pt |
dc.relation | fellowships from Fundação para a Ciência e Tecnologia | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Arachidonic Acids | pt |
dc.subject.mesh | Basic Helix-Loop-Helix Transcription Factors | pt |
dc.subject.mesh | Cell Differentiation | pt |
dc.subject.mesh | Cell Proliferation | pt |
dc.subject.mesh | Cells, Cultured | pt |
dc.subject.mesh | Cerebral Ventricles | pt |
dc.subject.mesh | GABAergic Neurons | pt |
dc.subject.mesh | Gene Expression Regulation | pt |
dc.subject.mesh | Immunohistochemistry | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Mice, Inbred C57BL | pt |
dc.subject.mesh | Multipotent Stem Cells | pt |
dc.subject.mesh | Nerve Tissue Proteins | pt |
dc.subject.mesh | Neurites | pt |
dc.subject.mesh | Neurons | pt |
dc.subject.mesh | Receptor, Cannabinoid, CB1 | pt |
dc.subject.mesh | Stem Cells | pt |
dc.subject.mesh | Neurogenesis | pt |
dc.title | Activation of type 1 cannabinoid receptor (CB1R) promotes neurogenesis in murine subventricular zone cell cultures | pt |
dc.type | article | - |
degois.publication.firstPage | e63529 | pt |
degois.publication.issue | 5 | pt |
degois.publication.title | PLoS ONE | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1371/journal.pone.0063529 | pt |
degois.publication.volume | 8 | pt |
dc.date.embargo | 2013-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-6854-2509 | - |
crisitem.author.orcid | https://orcid.org/0000-0003-0237-499X | - |
crisitem.author.orcid | 0000-0002-5438-4447 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Activation-of-Type-1-Cannabinoid-Receptor-CB1R-Promotes-Neurogenesis-in-Murine-Subventricular-Zone-Cell-CulturesPLoS-ONE.pdf | 2.81 MB | Adobe PDF | View/Open |
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