Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/109269
DC FieldValueLanguage
dc.contributor.authorRodrigues, Ana Sofia-
dc.contributor.authorCorreia, Marcelo-
dc.contributor.authorGomes, Andreia-
dc.contributor.authorPereira, Sandro L.-
dc.contributor.authorPerestrelo, Tânia-
dc.contributor.authorSousa, Maria Inês-
dc.contributor.authorRamalho-Santos, João-
dc.date.accessioned2023-10-06T10:33:59Z-
dc.date.available2023-10-06T10:33:59Z-
dc.date.issued2015-
dc.identifier.issn1932-6203pt
dc.identifier.urihttps://hdl.handle.net/10316/109269-
dc.description.abstractThe pyruvate dehydrogenase (PDH) complex is localized in the mitochondrial matrix catalyzing the irreversible decarboxylation of pyruvate to acetyl-CoA and NADH. For proper complex regulation the E1-α subunit functions as an on/off switch regulated by phosphorylation/dephosphorylation. In different cell types one of the four-pyruvate dehydrogenase kinase isoforms (PDHK1-4) can phosphorylate this subunit leading to PDH inactivation. Our previous results with human Embryonic Stem Cells (hESC), suggested that PDHK could be a key regulator in the metabolic profile of pluripotent cells, as it is upregulated in pluripotent stem cells. Therefore, we wondered if metabolic modulation, via inexpensive pharmacological inhibition of PDHK, could impact metabolism and pluripotency.pt
dc.language.isoengpt
dc.publisherPublic Library of Sciencept
dc.relationThe authors thank Fundação para a Ciência e a Tecnologia (FCT) Portugal for grant support (PTDC/EBB-EBI/ 120634/2010 and PDTC/ QUI-BIQ/120652/2010 co-funded by Compete/ FEDER/National Funds; and scholarships attributed to ASR (SFRH/BD/33463/2008); to MC (SFRH/BD/ 51681/2011), to TP (SFRH/BD/51684/2011), AG (SFRH/BD/51968/2012) and SLP (SFRH/BPD/98995/ 2013) and also to QREN (Centro-01-0762-FEDER- 00204). Center for Neuroscience and Cell Biology (CNC) funding is also supported by FCT (PEst-C/ SAU/LA0001/2011)pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshAnimalspt
dc.subject.meshCell Linept
dc.subject.meshCell Proliferationpt
dc.subject.meshDichloroacetic Acidpt
dc.subject.meshEmbryonic Stem Cellspt
dc.subject.meshGlycolysispt
dc.subject.meshMicept
dc.subject.meshOxidative Phosphorylationpt
dc.subject.meshPluripotent Stem Cellspt
dc.subject.meshPyruvate Dehydrogenase Complexpt
dc.titleDichloroacetate, the Pyruvate Dehydrogenase Complex and the Modulation of mESC Pluripotencypt
dc.typearticle-
degois.publication.firstPagee0131663pt
degois.publication.issue7pt
degois.publication.titlePLoS ONEpt
dc.peerreviewedyespt
dc.identifier.doi10.1371/journal.pone.0131663pt
degois.publication.volume10pt
dc.date.embargo2015-01-01*
uc.date.periodoEmbargo0pt
item.cerifentitytypePublications-
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-7060-879X-
crisitem.author.orcid0000-0001-9498-3965-
crisitem.author.orcid0000-0002-1172-4018-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
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