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https://hdl.handle.net/10316/109183
Title: | Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics | Authors: | Coelho, Margarida Nunes, Patricia Mendes, Vera M. Manadas, Bruno Heerschap, Arend Jones, John G. |
Issue Date: | 2015 | Publisher: | Hindawi | Project: | PTDC/SAU-MET/111398/2009 EXCL/DTP-PIC/0069/2012 PEst-C/SAU/LA0001/2013 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013/PT |
Serial title, monograph or event: | Journal of Diabetes Research | Volume: | 2015 | Abstract: | Mice deficient in adipose triglyceride lipase (ATGL(-/-)) present elevated ectopic lipid levels but are paradoxically glucose-tolerant. Measurement of endogenous glucose production (EGP) and Cori cycle activity provide insights into the maintenance of glycemic control in these animals. These parameters were determined in 7 wild-type (ATGL(+/-)) and 6 ATGL(-/-) mice by a primed-infusion of [U-(13)C6]glucose followed by LC-MS/MS targeted mass-isotopomer analysis of blood glucose. EGP was quantified by isotope dilution of [U-(13)C6]glucose while Cori cycling was estimated by analysis of glucose triose (13)C-isotopomers. Fasting plasma free fatty-acids were significantly lower in ATGL(-/-) versus control mice (0.43 ± 0.05 mM versus 0.73 ± 0.11 mM, P < 0.05). Six-hour fasting EGP rates were identical for both ATGL(-/-) and control mice (79 ± 11 versus 71 ± 7 μmol/kg/min, resp.). Peripheral glucose metabolism was dominated by Cori cycling (80 ± 2% and 82 ± 7% of glucose disposal for ATGL(-/-) and control mice, resp.) indicating that peripheral glucose oxidation was not significantly upregulated in ATGL(-/-) mice under these conditions. The glucose (13)C-isotopomer distributions in both ATGL(-/-) and control mice were consistent with extensive hepatic pyruvate recycling. This suggests that gluconeogenic outflow from the Krebs cycle was also well compensated in ATGL(-/-) mice. | URI: | https://hdl.handle.net/10316/109183 | ISSN: | 2314-6745 2314-6753 |
DOI: | 10.1155/2015/542029 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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Effect-of-Global-ATGL-Knockout-on-Murine-Fasting-Glucose-KineticsJournal-of-Diabetes-Research.pdf | 1.36 MB | Adobe PDF | View/Open |
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