Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/109147
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dc.contributor.authorMachado, Vanessa M.-
dc.contributor.authorMorte, Maria I.-
dc.contributor.authorCarreira, Bruno P.-
dc.contributor.authorAzevedo, Maria M.-
dc.contributor.authorTakano, Jiro-
dc.contributor.authorIwata, Nobuhisa-
dc.contributor.authorSaido, Takaomi C.-
dc.contributor.authorAsmussen, Hannelore-
dc.contributor.authorHorwitz, Alan R.-
dc.contributor.authorCarvalho, Caetana M.-
dc.contributor.authorAraújo, Inês M.-
dc.date.accessioned2023-09-29T08:01:43Z-
dc.date.available2023-09-29T08:01:43Z-
dc.date.issued2015-
dc.identifier.issn1662-5102pt
dc.identifier.urihttps://hdl.handle.net/10316/109147-
dc.description.abstractCalpains are ubiquitous proteases involved in cell proliferation, adhesion and motility. In the brain, calpains have been associated with neuronal damage in both acute and neurodegenerative disorders, but their physiological function in the nervous system remains elusive. During brain ischemia, there is a large increase in the levels of intracellular calcium, leading to the activation of calpains. Inhibition of these proteases has been shown to reduce neuronal death in a variety of stroke models. On the other hand, after stroke, neural stem cells (NSC) increase their proliferation and newly formed neuroblasts migrate towards the site of injury. However, the process of forming new neurons after injury is not efficient and finding ways to improve it may help with recovery after lesion. Understanding the role of calpains in the process of neurogenesis may therefore open a new window for the treatment of stroke. We investigated the involvement of calpains in NSC proliferation and neuroblast migration in two highly neurogenic regions in the mouse brain, the dentate gyrus (DG) and the subventricular zone (SVZ). We used mice that lack calpastatin, the endogenous calpain inhibitor, and calpains were also modulated directly, using calpeptin, a pharmacological calpain inhibitor. Calpastatin deletion impaired both NSC proliferation and neuroblast migration. Calpain inhibition increased NSC proliferation, migration speed and migration distance in cells from the SVZ. Overall, our work suggests that calpains are important for neurogenesis and encourages further research on their neurogenic role. Prospective therapies targeting calpain activity may improve the formation of new neurons following stroke, in addition to affording neuroprotection.pt
dc.language.isoengpt
dc.publisherFrontiers Media S.A.pt
dc.relationPTDC/SAU-NMC/112183/2009pt
dc.relationPEst-C/SAU/LA0001/2013-2014pt
dc.relationPEst-OE/EQB/LA0023/2013-2014pt
dc.relationNIHgrantGM23244pt
dc.relationSFRH/BPD/78901/2011pt
dc.relationSFRH/BD/38127/2007pt
dc.relationSFRH/BD/78050/2011pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectcalpainspt
dc.subjectcalpastatinpt
dc.subjecthippocampuspt
dc.subjectmigrationpt
dc.subjectneurogenesispt
dc.subjectproliferationpt
dc.subjectstrokept
dc.subjectsubventricular zonept
dc.titleInvolvement of calpains in adult neurogenesis: implications for strokept
dc.typearticle-
degois.publication.firstPage22pt
degois.publication.issueFEBpt
degois.publication.titleFrontiers in Cellular Neurosciencept
dc.peerreviewedyespt
dc.identifier.doi10.3389/fncel.2015.00022pt
degois.publication.volume9pt
dc.date.embargo2015-01-01*
uc.date.periodoEmbargo0pt
item.cerifentitytypePublications-
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-7874-6545-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
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This item is licensed under a Creative Commons License Creative Commons