Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/108912
DC FieldValueLanguage
dc.contributor.authorCardoso, Joana M. S.-
dc.contributor.authorAnjo, Sandra I.-
dc.contributor.authorFonseca, Luís-
dc.contributor.authorEgas, Conceição-
dc.contributor.authorManadas, Bruno-
dc.contributor.authorAbrantes, Isabel-
dc.date.accessioned2023-09-25T08:41:51Z-
dc.date.available2023-09-25T08:41:51Z-
dc.date.issued2016-12-12-
dc.identifier.issn2045-2322pt
dc.identifier.urihttps://hdl.handle.net/10316/108912-
dc.description.abstractThe pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS. From the 681 proteins initially identified, 422 were quantified and compared between B. xylophilus and B. mucronatus secretomes and from these, 243 proteins were found differentially regulated: 158 and 85 proteins were increased in B. xylophilus and B. mucronatus secretomes, respectively. While increased proteins in B. xylophilus secretome revealed a strong enrichment in proteins with peptidase activity, the increased proteins in B. mucronatus secretome were mainly related to oxidative stress responses. The changes in peptidases were evaluated at the transcription level by RT-qPCR, revealing a correlation between the mRNA levels of four cysteine peptidases with secretion levels. The analysis presented expands our knowledge about molecular basis of B. xylophilus and B. mucronatus hosts interaction and supports the hypothesis of a key role of secreted peptidases in B. xylophilus pathogenicity.pt
dc.language.isoengpt
dc.relationThis research was financed by Portuguese Foundation for Science and Technology (FCT) through national funds and the co-funding by FEDER- Fundo Europeu de Desenvolvimento Regional and COMPETEPrograma Operacional Factores de Competitividade, under the projects FCT PTDC/AGR-CFL/098916/2008 and FCT PTDC/NEU-NMC/0205/2012, within the PT2020 Partnership Agreement and COMPETE 2020 under the projects UID/BIA/04004/2013 and UID/NEU/04539/2013, the National Mass Spectrometry Network (RNEM, REDE/1506/REM/2005) and Instituto do Ambiente, Tecnologia e Vida, FCTUC. Joana M.S. Cardoso (SFRH/BPD/73724/2010) and Sandra I. Anjo (SFRH/BD/81495/2011) are funded by post-doctoral/ doctoral fellowships financed by QREN-POPH-Typology 4.1-co-financed by MES national funding and The European Social Fund. Luís Fonseca (SFRH/BPD/101325/2014) is funded by post-doctoral fellowship financed by MEC national funding and by the European Social Fund through POCH (Programa Operacional Capital Humano).pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshAnimalspt
dc.subject.meshGene Ontologypt
dc.subject.meshNematodapt
dc.subject.meshOxidative Stresspt
dc.subject.meshProteomicspt
dc.subject.meshTranscriptomept
dc.titleBursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysispt
dc.typearticle-
degois.publication.firstPage39007pt
degois.publication.issue1pt
degois.publication.titleScientific Reportspt
dc.peerreviewedyespt
dc.identifier.doi10.1038/srep39007pt
degois.publication.volume6pt
dc.date.embargo2016-12-12*
uc.date.periodoEmbargo0pt
item.grantfulltextopen-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextCom Texto completo-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.researchunitCFE - Centre for Functional Ecology - Science for People & the Planet-
crisitem.author.orcid0000-0002-6594-4892-
crisitem.author.orcid0000-0001-7405-8916-
crisitem.author.orcid0000-0002-2307-5414-
crisitem.author.orcid0000-0002-2087-4042-
crisitem.author.orcid0000-0002-8761-2151-
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais
Show simple item record

Page view(s)

65
checked on May 15, 2024

Download(s)

26
checked on May 15, 2024

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons