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Title: Short communication: The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
Authors: Pereira, Cátia 
Montalbán, Itziar A.
Goicoa, Tomás
Ugarte, M. Dolores
Correia, Sandra 
Canhoto, Jorge 
Moncaleán, Paloma
Keywords: embryogenic cell lines; Pinus halepensis; somatic embryos; water availability
Issue Date: 2018
Publisher: Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria
Project: MINECO 
Spanish Government (project AGL2013-4700-C4-2R) 
Serial title, monograph or event: Forest Systems
Volume: 26
Issue: 3
Abstract: Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability.
ISSN: 2171-9845
DOI: 10.5424/fs/2017263-11436
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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