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Title: Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
Authors: Dong, Lan-Feng
Kovarova, Jaromira 
Bajzikova, Martina 
Bezawork-Geleta, Ayenachew 
Svec, David 
Endaya, Berwini 
Sachaphibulkij, Karishma
Coelho, Ana R. 
Sebkova, Natasa
Ruzickova, Anna
Tan, An S
Kluckova, Katarina 
Judasova, Kristyna 
Zamecnikova, Katerina
Rychtarcikova, Zuzana
Gopalan, Vinod
Andera, Ladislav
Sobol, Margarita 
Yan, Bing
Pattnaik, Bijay
Bhatraju, Naveen
Truksa, Jaroslav 
Stopka, Pavel
Hozak, Pavel 
Lam, Alfred K.
Sedlacek, Radislav
Oliveira, Paulo J. 
Kubista, Mikael 
Agrawal, Anurag
Dvorakova-Hortova, Katerina 
Rohlena, Jakub 
Berridge, Michael V. 
Neuzil, Jiri 
Keywords: cancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growth
Issue Date: 15-Feb-2017
Publisher: eLife Sciences Publications
Project: Funder Grant reference number Author Fundac¸a˜o para a Cieˆ ncia e a Tecnologia SFRH/BD/103399/2014 Ana R Coelho Cancer Society of New Zealand An S Tan Michael V Berridge Genesis Oncology Trust An S Tan Michael V Berridge Malaghan Institute of Medical Research An S Tan Michael V Berridge Council for Scientific and In- dustrial Research MLP5502 Anurag Agrawal Naveen Bhatraju Bijay Pattnaik Wellcome DBT India Alliance Senior Fellowship Bijay Pattnaik Naveen Bhatraju Anurag Agrawal Czech Science Foundation 16-12816S Jaroslav Truksa Ministerstvo Sˇ kolstvı´, Mla´ dezˇe a Teˇ lovy´chovy LM2011032 Radislav Sedlacek Akademie veˇ d Cˇ eske´ republiky RVO 68378050 Radislav Sedlacek Czech Science Foundation 14-05547S Katerina Dvorakova-Hortova Czech Science Foundation 16-22823S Jakub Rohlena Czech Science Foundation 17–20904S Jakub Rohlena Czech Science Foundation 16–12816S Jakub Rohlena Australian Research Council DP150102820 Jiri Neuzil Czech Science Foundation 17–0192J Jiri Neuzil Czech Science Foundation 16-12719S Jiri Neuzil Czech Science Foundation 15-02203S Jiri Neuzil BIOCEV European Regional Development Jiri Neuzil 
Serial title, monograph or event: eLife
Volume: 6
Abstract: Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.
ISSN: 2050-084X
DOI: 10.7554/eLife.22187
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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