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https://hdl.handle.net/10316/108044
Title: | An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide | Authors: | Simões, Tiago Novais, Sara C Luz, Tiago Natal da Devreese, Bart de Boer, Tjalf Roelofs, Dick Sousa, José P. van Straalen, Nico M Lemos, Marco F. L. |
Issue Date: | 27-Jul-2018 | Publisher: | Springer Nature | Project: | UID/MAR/04292/2013 PTDC/AGR-PRO/3496/2012 POCI-01-0145-FEDER-016773 SFRH/ BD/98266/2013 SFRH/BPD/94500/2013 SFRH/BPD/110943/2015 BE-BASIC project F08.001.03 EU FP7 Sustainable Nanotechnologies project (SUN, grant number 604305) |
Serial title, monograph or event: | Scientific Reports | Volume: | 8 | Issue: | 1 | Abstract: | The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87-1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction. | URI: | https://hdl.handle.net/10316/108044 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-29662-6 | Rights: | openAccess |
Appears in Collections: | I&D CFE - Artigos em Revistas Internacionais |
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