Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/107982
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Da Costa, Raul | - |
dc.contributor.author | Amaral, Sandra | - |
dc.contributor.author | Redmann, Klaus | - |
dc.contributor.author | Kliesch, Sabine | - |
dc.contributor.author | Schlatt, Stefan | - |
dc.date.accessioned | 2023-08-03T10:18:21Z | - |
dc.date.available | 2023-08-03T10:18:21Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1932-6203 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/107982 | - |
dc.description.abstract | Raman Microspectroscopy represents an innovative tool for the assessment of sperm biochemical features otherwise undetectable by routine semen analysis. Previously, it was shown that induced DNA damage can be detected in smeared sperm by this technique. This novel readout may be of value for clinical settings especially if it can be transferred to living cells. Yet, starting with living sperms this study was carried-out using a variety of conditions to disclose the Raman features of sperm nuclei under different hydration conditions and UV exposure. Human sperm were immobilized and Raman spectra were obtained from individual sperm as repeated measurements. To create conditions with controlled DNA damage, sperm samples were exposed to ultraviolet light. Several media were used to evaluate their effect on Raman spectra in aqueous conditions. To substantiate differences between the experimental conditions, the spectra were analyzed by Principal Component Analysis. We observed that spectra of sperm nuclei obtained in different solutions showed a qualitatively unchanged spectral pattern showing the principal signals related to DNA. Evaluating the effect of ultraviolet light generated the finding that spectra representing DNA damage were only observed in dry conditions but not in aqueous medium. Thus, Raman microspectroscopy was successfully applied for sperm analysis in different conditions, among them in live spermatozoa in aqueous solution during the initial measurement, revealing the principle use of this technique. However, implementation of Raman spectroscopy as a technique for clinical sperm analysis and selection may be especially relevant when DNA evaluation can be established using live sperm. | pt |
dc.language.iso | eng | pt |
dc.publisher | Public Library of Science | pt |
dc.relation | DAAD PhD stipend (91590556) | pt |
dc.relation | FCT - SFRH/BPD/110160/2015 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Cell Nucleus | pt |
dc.subject.mesh | DNA | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Male | pt |
dc.subject.mesh | Spermatozoa | pt |
dc.subject.mesh | Water | pt |
dc.subject.mesh | Spectrum Analysis, Raman | pt |
dc.subject.mesh | Ultraviolet Rays | pt |
dc.title | Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration | pt |
dc.type | article | - |
degois.publication.firstPage | e0207786 | pt |
degois.publication.issue | 11 | pt |
degois.publication.title | PLoS ONE | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1371/journal.pone.0207786 | pt |
degois.publication.volume | 13 | pt |
dc.date.embargo | 2018-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-5542-7763 | - |
Appears in Collections: | IIIUC - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Spectral-features-of-nuclear-DNA-in-human-sperm-assessed-by-Raman-Microspectroscopy-Effects-of-UVirradiation-and-hydrationPLoS-ONE.pdf | 2.09 MB | Adobe PDF | View/Open |
Page view(s)
47
checked on Aug 14, 2024
Download(s)
28
checked on Aug 14, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License