Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/107980
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dc.contributor.authorSaraiva, Cláudia-
dc.contributor.authorTalhada, Daniela-
dc.contributor.authorRai, Akhilesh-
dc.contributor.authorFerreira, Raquel-
dc.contributor.authorFerreira, Lino-
dc.contributor.authorBernardino, Liliana-
dc.contributor.authorRuscher, Karsten-
dc.date.accessioned2023-08-03T09:55:44Z-
dc.date.available2023-08-03T09:55:44Z-
dc.date.issued2018-
dc.identifier.issn1932-6203pt
dc.identifier.urihttps://hdl.handle.net/10316/107980-
dc.description.abstractThere is a high quest for novel therapeutic strategies to enhance recovery after stroke. MicroRNA-124 (miR-124) has been described as neuroprotective and anti-inflammatory molecule. Moreover, miR-124 is a well described enhancer of adult neurogenesis that could offer potentially beneficial effects. Herein, we used miR-124-loaded nanoparticles (miR-124 NPs) to evaluate their therapeutic potential in an in vitro and in vivo model of stroke. For that, neuroprotective and neurogenic responses were assessed in an in vitro model of stroke. Here, we found that miR-124 NPs decreased cell death and improved neuronal differentiation of subventricular zone (SVZ) neural stem cell cultures after oxygen and glucose deprivation. In contrast, intravenous injection of miR-124 NPs immediately after permanent focal ischemia induced by photothrombosis (PT) did not provide a better neurological outcome. In addition, treatment did not affect the number of 5-bromo-2'-deoxyuridine (BrdU)- and doublecortin/BrdU- positive cells in the SVZ at the study endpoint of 14 days after PT. Likewise, the ischemic insult did not affect the numbers of neuronal progenitors in the SVZ. However, in PT mice miR-124 NPs were able to specifically augment interleukin-6 levels at day 2 post-stroke. Furthermore, we also showed that NPs reached the brain parenchyma and were internalized by brain resident cells. Although, promising in vitro data could not be verified in vivo as miR-124 NPs treatment did not improve functional outcome nor presented beneficial actions on neurogenesis or post-stroke inflammation, we showed that our NP formulation can be a safe alternative for drug delivery into the brain.pt
dc.language.isoengpt
dc.publisherPublic Library of Sciencept
dc.relationPOCI-01-0145-FEDER-007491pt
dc.relationSFRH/BD/90365/2012pt
dc.relationUID/Multi /00709/2013)pt
dc.relationSwedish Brain Fundpt
dc.relationCrafoord Foundationpt
dc.relationSwedish Research Council,pt
dc.relationSveriges Stroke RiksfoÈrbundetpt
dc.relationHans-Christian and Alice Wachtmeister Foundationpt
dc.relationStiftelsen Sven- Olof Jansons livsverkpt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshAdministration, Intravenouspt
dc.subject.meshAnimalspt
dc.subject.meshApoptosispt
dc.subject.meshBrain Ischemiapt
dc.subject.meshCell Differentiationpt
dc.subject.meshCell Proliferationpt
dc.subject.meshCells, Culturedpt
dc.subject.meshDisease Models, Animalpt
dc.subject.meshInterleukin-6pt
dc.subject.meshMalept
dc.subject.meshMicept
dc.subject.meshMicroRNAspt
dc.subject.meshNanoparticlespt
dc.subject.meshNeural Stem Cellspt
dc.subject.meshStrokept
dc.subject.meshTreatment Outcomept
dc.titleMicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivopt
dc.typearticle-
degois.publication.firstPagee0193609pt
degois.publication.issue3pt
degois.publication.titlePLoS ONEpt
dc.peerreviewedyespt
dc.identifier.doi10.1371/journal.pone.0193609pt
degois.publication.volume13pt
dc.date.embargo2018-01-01*
uc.date.periodoEmbargo0pt
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.cerifentitytypePublications-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-5352-5415-
crisitem.author.orcid0000-0001-8985-9302-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
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This item is licensed under a Creative Commons License Creative Commons